Huang Long, Pei Tianchu, Wu Guohui, Liu Jiamin, Pan Wenna, Pan Xuan
Resident, The First Affiliated Hospital/Clinical Medical School, Guangdong Pharmaceutical University, Guangzhou, Guangdong, China.
Professor, The First Affiliated Hospital/Clinical Medical School, Guangdong Pharmaceutical University, Guangzhou, Guangdong, China.
J Oral Maxillofac Surg. 2022 Apr;80(4):756-766. doi: 10.1016/j.joms.2021.11.021. Epub 2021 Dec 4.
Studies have found a positive correlation between various cancers and circular RNAs (circRNAs), which are newly discovered noncoding RNAs. However, limited scientific evidence is available to prove the clinical value of circRNAs in the presentation of oral squamous cell carcinoma (OSCC). This study aimed to explore comprehensively the potential of circRNAs as diagnostic indexes of OSCC.
Online databases were systematically searched to identify published literature on the discovery of circRNAs in OSCC. Data were acquired from each reviewed study and collated to create a 2 × 2 eventuality table. Hierarchical analysis of the literature was conducted for the type of cancer, year of publication, and the sample size of each study. The diagnostic accuracy was calculated using indexes such as the pooled sensitivity and specificity, and assessed critically using the Quality Assessment for Studies of Diagnostic Accuracy 2.
This meta-analysis included findings of 6 studies on 335 patients diagnosed with OSCC. These 6 studies examined 7 circRNAs, 5 in tissues and 2 in the saliva of patients with OSCC. When used as a diagnostic tool for OSCC, circRNAs manifested a sensitivity level of 0.72 (95% confidence interval: 0.67 to 0.76) and a degree of specificity of 0.81 (95% confidence interval: 0.76 to 0.85), with a general projected probability rate of 3.82 (95% confidence interval: 2.98 to 4.91) being positive and 0.35 (95% confidence interval: 0.29 to 0.41) being negative. The combined probability rate was 11.07 (95% confidence interval: 7.64 to 16.04), comprising a total of 0.76 (95% confidence interval: 0.72 to 0.79) of the region under the curve. A higher diagnostic value was found for salivary circRNAs (diagnostic odds ratio = 17.52; 95% CI: 10.11 to 30.35) than for tissue circRNAs (diagnostic odds ratio = 8.47; 95% CI: 5.6 to 12.83). This indicated that circRNAs showed a good discrimination ability as biomarkers of OSCC.
circRNAs showed high accuracy in the diagnosis of OSCC and could be used as prospective biomarkers to facilitate the diagnostic process.
研究发现多种癌症与环状RNA（circRNA）之间存在正相关，环状RNA是新发现的非编码RNA。然而，目前仅有有限的科学证据能够证明circRNA在口腔鳞状细胞癌（OSCC）表现中的临床价值。本研究旨在全面探索circRNA作为OSCC诊断指标的潜力。
系统检索在线数据库，以识别已发表的关于在OSCC中发现circRNA的文献。从每项纳入综述的研究中获取数据并进行整理，以创建一个2×2列联表。对文献进行分层分析，涉及癌症类型、发表年份以及每项研究的样本量。使用合并敏感性和特异性等指标计算诊断准确性，并使用诊断准确性研究质量评估2进行严格评估。
这项荟萃分析纳入了6项关于335例被诊断为OSCC患者的研究结果。这6项研究检测了7种circRNA，其中5种在OSCC患者的组织中检测，2种在唾液中检测。当用作OSCC的诊断工具时，circRNA表现出的敏感性水平为0.72（95%置信区间：0.67至0.76），特异性程度为0.81（95%置信区间：0.76至0.85），总体预测阳性概率率为3.82（95%置信区间：2.98至4.91），阴性概率率为0.35（95%置信区间：0.29至0.41）。合并概率率为11.07（95%置信区间：7.64至16.04），曲线下面积总计为0.76（95%置信区间：0.72至0.79）。发现唾液circRNA的诊断价值高于组织circRNA（诊断比值比 = 17.52；95% CI：10.11至30.35）（诊断比值比 = 8.47；95% CI：5.6至12.83）。这表明circRNA作为OSCC的生物标志物具有良好的鉴别能力。
circRNA在OSCC诊断中显示出高准确性，可作为前瞻性生物标志物以促进诊断过程。
