免疫细胞的柱分级分离研究。VII. 在弱碱性离子交换剂上分级分离后小鼠脾细胞的电泳迁移率和功能行为。
Studies on column fractionation of immune cells. VII. Electrophoretic mobility and functional behavior of mouse spleen cells after fractionation on a weakly basic ion exchanger.
作者信息
Eckert R, Jenssen H L, Köhler H, von Broen B, Pasternak G
出版信息
Acta Biol Med Ger. 1978;37(9):1393-7.
After passing normal mouse spleen cells through columns of a weakly basic ion-exchanger, the percentage of cells with B cell-typical electrophoretic mobility (EPM) is 90% after fractionation at pH 8.0 and 10--20% at pH 5.0. However, the characterization of fractionated cells with regard to theta-antigen as well as graft-versus-host reaction shows that separation of T- and B-lymphocytes had not occurred. After incubation of the separated cells for 2 h at 37 degrees C the EPM returned to that of unseparated cells. It appears that the net charge of T- and B-lymphocytes is changeable depending on pH, temperature and separation material.
将正常小鼠脾细胞通过弱碱性离子交换柱后，在pH 8.0分级分离时，具有B细胞典型电泳迁移率（EPM）的细胞百分比为90%，而在pH 5.0时为10%-20%。然而，对分级分离细胞进行θ抗原以及移植物抗宿主反应的表征表明，T淋巴细胞和B淋巴细胞并未分离。将分离的细胞在37℃孵育2小时后，其EPM恢复到未分离细胞的水平。似乎T淋巴细胞和B淋巴细胞的净电荷会根据pH、温度和分离材料而变化。
Zotero 插件