Wiese-Rischke Cornelia, Murkar Rasika S, Walles Heike
University Clinic for Cardio and Thoracic Surgery, Otto-von-Guericke-University Magdeburg, Leipziger Str. 44, 39120 Magdeburg, Germany.
Core Facility Tissue Engineering, Institute of Chemistry, Otto-von-Guericke-University Magdeburg, Pfaelzer Str. 2, 39106 Magdeburg, Germany.
Pharmaceutics. 2021 Dec 8;13(12):2115. doi: 10.3390/pharmaceutics13122115.
In our review, we want to summarize the current status of the development of airway models and their application in biomedical research. We start with the very well characterized models composed of cell lines and end with the use of organoids. An important aspect is the function of the mucus as a component of the barrier, especially for infection research. Finally, we will explain the need for a nondestructive characterization of the barrier models using TEER measurements and live cell imaging. Here, organ-on-a-chip technology offers a great opportunity for the culture of complex airway models.
在我们的综述中，我们想要总结气道模型的发展现状及其在生物医学研究中的应用。我们从由细胞系组成的特征明确的模型开始，以类器官的应用结束。一个重要方面是黏液作为屏障组成部分的功能，特别是在感染研究方面。最后，我们将解释使用跨上皮电阻（TEER）测量和活细胞成像对屏障模型进行无损表征的必要性。在这里，芯片上器官技术为复杂气道模型的培养提供了一个绝佳机会。
