Sharma Monita, Huber Erin, Arnesdotter Emma, Behrsing Holger P, Bettmann Adam, Brandwein David, Constant Samuel, Date Rahul, Deshpande Abhay, Fabian Eric, Gupta Amit, Gutierrez Robert, Gutleb Arno C, Hargrove Marie M, Hollings Michael, Hutter Victoria, Jarabek Annie M, Kaluzhny Yulia, Landsiedel Robert, Milchak Lawrence, Moyer Robert A, Murray Jessica R, Page Kathryn, Patel Manish, Pearson Stephanie N, Petersen Elijah J, Reinke Emily, Roldan Nuria, Roper Clive, Scaglione Jamie B, Settivari Raja S, Stucki Andreas O, Verstraelen Sandra, Wallace Joanne L, McCullough Shaun, Clippinger Amy J
PETA Science Consortium International e.V., 70499, Stuttgart, Germany.
Exposure and Protection, RTI International, 3040 East Cornwallis Road, Durham, NC, USA.
Arch Toxicol. 2025 Jan;99(1):57-66. doi: 10.1007/s00204-024-03879-z. Epub 2024 Oct 4.
Standard information reporting helps to ensure that assay conditions and data are consistently reported and to facilitate inter-laboratory comparisons. Here, we present recommendations on minimum information for reporting on the TEER (trans-epithelial/endothelial electrical resistance) assay (MIRTA). The TEER assay is extensively used to evaluate the health of an epithelial/endothelial cell culture model and as an indicator of the potential toxicity of a test substance. This publication is the result of an international collaboration─called the RespTox (Respiratory Toxicity) Collaborative─through which twelve laboratories shared their protocols for assessing the barrier function of respiratory epithelial cells using the TEER assay following exposure to substances. The protocols from each laboratory were reviewed to identify general steps for performing the TEER assay, interlaboratory differences between steps, the rationale for differences, whether these differences impact results or cross-laboratory comparisons between TEER measurements. While the MIRTA recommendations are focused on respiratory epithelial cell systems, these recommendations can be adapted for other cell systems that form barriers. The use of these recommendations will support data transparency and reproducibility, reduce challenges in data interpretation, enable cross-laboratory comparisons, help assess study quality, and facilitate the incorporation of the TEER assay into national and international testing guidance.
标准信息报告有助于确保一致地报告检测条件和数据，并便于实验室间的比较。在此，我们提出关于跨上皮/内皮电阻（TEER）检测报告的最低限度信息（MIRTA）的建议。TEER检测被广泛用于评估上皮/内皮细胞培养模型的健康状况，并作为测试物质潜在毒性的指标。本出版物是一项国际合作——称为RespTox（呼吸毒性）合作项目——的成果，通过该项目，十二个实验室分享了他们在接触物质后使用TEER检测评估呼吸道上皮细胞屏障功能的方案。对每个实验室的方案进行了审查，以确定进行TEER检测的一般步骤、步骤之间的实验室间差异、差异的原理、这些差异是否会影响结果或TEER测量之间的跨实验室比较。虽然MIRTA建议侧重于呼吸道上皮细胞系统，但这些建议可适用于形成屏障的其他细胞系统。使用这些建议将支持数据透明度和可重复性，减少数据解释中的挑战，实现跨实验室比较，有助于评估研究质量，并促进将TEER检测纳入国家和国际测试指南。
