Giordano Bryan V, Cruz Anthony, Pérez-Ramos Daniel W, Ramos Martina M, Tavares Yasmin, Caragata Eric P
Florida Medical Entomology Laboratory, Department of Entomology and Nematology, Institute of Food and Agriculture, University of Florida, Vero Beach, FL 32962, USA.
Pathogens. 2021 Dec 3;10(12):1575. doi: 10.3390/pathogens10121575.
Mosquito and arbovirus surveillance is essential to the protection of public health. A majority of surveys are undertaken at ground level. However, mosquitoes shelter, breed, and quest for hosts across vertical strata, thus limiting our ability to fully describe mosquito and arboviral communities. To elucidate patterns of mosquito vertical stratification, canopy traps were constructed to sample mosquitoes at heights of 1.5, 5.0, and 8.7 m across three different landscape types in a Florida coastal conservation area. We assessed trapping efforts using individual-based rarefaction and extrapolation. The effects of height, landscape, site location, and sampling date on mosquito community composition were parsed out using permutational ANOVA on a Hellinger-transformed Bray-Curtis dissimilarity abundance matrix. Lastly, a generalized linear mixed effects model (GLMM) was used to explore species-specific vertical patterns. We observed differences in sampling effort and community composition structure across various heights and landscapes. Our GLMM revealed significant effects of trap height for , , , and but not for , the ultra-dominant species present in this area. Together these data provide evidence that height and landscape significantly affect mosquito community structures and highlight a need to develop sampling regimes to target specific vector and nuisance species at their preferred height and across different landscape types.
蚊子和虫媒病毒监测对于保护公众健康至关重要。大多数调查是在地面进行的。然而，蚊子在垂直分层中栖息、繁殖并寻找宿主，因此限制了我们全面描述蚊子和虫媒病毒群落的能力。为了阐明蚊子垂直分层模式，在佛罗里达沿海保护区的三种不同景观类型中，设置了树冠陷阱，在1.5米、5.0米和8.7米的高度对蚊子进行采样。我们使用基于个体的稀疏化和外推法评估诱捕效果。使用基于排列的方差分析对经Hellinger变换的Bray-Curtis差异丰度矩阵，分析高度、景观、地点位置和采样日期对蚊子群落组成的影响。最后，使用广义线性混合效应模型（GLMM）探索特定物种的垂直模式。我们观察到不同高度和景观的采样效果和群落组成结构存在差异。我们的GLMM显示诱捕高度对[具体物种1]、[具体物种2]、[具体物种3]和[具体物种4]有显著影响，但对该地区的超优势物种[具体物种5]没有显著影响。这些数据共同表明，高度和景观显著影响蚊子群落结构，并强调需要制定采样方案，以在其偏好的高度和不同景观类型中针对特定的病媒和滋扰物种。
