Faculty of Food Science and Engineering, Kunming University of Science and Technology, Kunming 650500, China.
School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
Nutrients. 2021 Dec 15;13(12):4480. doi: 10.3390/nu13124480.
Hepatic glycolipid metabolism disorder is considered as one of the key factors in the pathogenesis of many chronic diseases. The objective of this study was to investigate the protective effect and underlying mechanisms of Mill. fruits against hepatic glycolipid metabolic disorders in rats induced by a high fat/high sugar diet. Results showed that ethanol extract, especially at a dose of 600 mg/kg b.w., could effectively ameliorate glycolipid metabolic disorders in rats. The biochemical indexes, including CAT, GSH and HOMA-IR, were significantly improved by the administration of ethanol extract. Immunohistochemistry and Western blot analysis revealed that ethanol extract up-regulated the expression levels of PI3K/AKT, PPAR-α, and the phosphorylation of IRS1 and AMPK proteins, and down-regulated the expressions of SREBP-1 and FAS proteins in the liver, which are closely related to hepatic glycolipid metabolism. Those findings suggested that Mill. fruits could be developed as functional foods and/or nutraceuticals for preventing or controlling some chronic diseases related to hepatic glycolipid metabolism disorder.
肝糖脂代谢紊乱被认为是许多慢性疾病发病机制中的关键因素之一。本研究旨在探讨密蒙花对高脂高糖饮食诱导的大鼠肝糖脂代谢紊乱的保护作用及其潜在机制。结果表明，密蒙花乙醇提取物，特别是剂量为 600mg/kg bw 时，能有效改善大鼠的糖脂代谢紊乱。生化指标如 CAT、GSH 和 HOMA-IR 经乙醇提取物处理后显著改善。免疫组化和 Western blot 分析表明，乙醇提取物能上调肝脏中 PI3K/AKT、PPAR-α 以及 IRS1 和 AMPK 蛋白磷酸化的表达水平，下调 SREBP-1 和 FAS 蛋白的表达，这些与肝糖脂代谢密切相关。这些发现表明，密蒙花可作为功能性食品和/或天然药物开发，用于预防或控制与肝糖脂代谢紊乱相关的某些慢性疾病。
