Enouf J, Giraud F, Bredoux R, Bourdeau N, Levy-Toledano S
Biochem Biophys Res Commun. 1987 May 29;145(1):139-45. doi: 10.1016/0006-291x(87)91298-8.
The effect of inositol 1,4,5 trisphosphate (IP3) has been investigated on the cAMP-induced phosphorylation of the 23-Kd protein involved in platelet calcium fluxes by isolated membrane vesicles. The studies were conducted using the catalytic subunit of the cAMP-dependent protein kinase (C. Sub.). A dose-dependent stimulation of the 23 Kd protein phosphorylation induced by C. Sub. was initiated by IP3 with a half-maximal effect of 0.5 microM. The maximal effect was observed after 1-2 min. The effect was detected in the absence of Ca2+ and in the presence of phosphatase inhibitors. These results can suggest that the 23 Kd is an associated protein to the IP3 receptor in human platelets.
通过分离的膜囊泡，研究了肌醇1,4,5-三磷酸（IP3）对参与血小板钙通量的23-Kd蛋白的cAMP诱导磷酸化的影响。研究使用了cAMP依赖性蛋白激酶的催化亚基（C.Sub.）进行。IP3引发了C.Sub.诱导的23 Kd蛋白磷酸化的剂量依赖性刺激，半最大效应为0.5 microM。1-2分钟后观察到最大效应。在不存在Ca2+和存在磷酸酶抑制剂的情况下检测到该效应。这些结果表明23 Kd是人类血小板中IP3受体的相关蛋白。
