Segura-Palacios Mario Alberto, Correa-Pacheco Zormy Nacary, Corona-Rangel Maria Luisa, Martinez-Ramirez Ollin Celeste, Salazar-Piña Dolores Azucena, Ramos-García Margarita de Lorena, Bautista-Baños Silvia
Facultad de Nutrición, Universidad Autónoma del Estado de Morelos, Calle Iztaccihuatl S/N, Col. Los Volcanes, Cuernavaca C.P. 62350, Mexico.
Centro de Desarrollo de Productos Bióticos, Instituto Politécnico Nacional, Carretera Yautepec-Jojutla, km. 6.8, San Isidro, CEPROBI 8, Yautepec C.P. 62731, Mexico.
Plants (Basel). 2021 Nov 23;10(12):2553. doi: 10.3390/plants10122553.
affects fresh and dry fruit and vegetable products, and its toxic metabolites, namely aflatoxins, cause serious damage in humans. The objective of this research study was to evaluate the effect of commercial natural products as well as edible and nanostructured chitosan coatings on the development of and on the production of aflatoxins in vitro and in tomato. Treatments were as follows: chitosan 1%, chitosan coating, chitosan nanostructured coating, Citrocover 1% (citrus seed extract), Resinadher 0.5% (pine resin extract), mancozeb 2%, and water. The variables were as follows: halo inhibition, spore production, and aflatoxins content. In fruit, the following were evaluated: disease incidence, mycelial growth, and aflatoxin production. An ANOVA (Tukey: < 0.05) was used. In vitro results showed that Citrocover and Resinadher reduced sporulation (0.2 and 0.9 × 10 spores mL, respectively), while chitosan inhibited the production of aflatoxins. With Resinadher and Citrocover, tomato fruit had the lowest incidence, mycelial growth, and aflatoxin production with corresponding values of 0%, 0.0 cm, and 0.95 ppb, respectively, and 7%, 0.2 cm, and 1.77 ppb, respectively. The use of Citrocover and Resinadher could be a viable alternative to decrease the development of in tomato fruit.
影响新鲜和干燥的水果及蔬菜产品，其有毒代谢产物即黄曲霉毒素会对人类造成严重损害。本研究的目的是评估商业天然产品以及可食用和纳米结构的壳聚糖涂层对体外和番茄中黄曲霉的生长以及黄曲霉毒素产生的影响。处理方式如下：1%壳聚糖、壳聚糖涂层、纳米结构壳聚糖涂层、1% Citrocover（柑橘籽提取物）、0.5% Resinadher（松脂提取物）、2%代森锰锌和水。变量如下：抑菌圈、孢子产生量和黄曲霉毒素含量。在果实中，评估了以下指标：发病率、菌丝生长和黄曲霉毒素产生量。使用了方差分析（Tukey检验：P < 0.05）。体外结果表明，Citrocover和Resinadher减少了孢子形成（分别为0.2和0.9×10个孢子/毫升），而壳聚糖抑制了黄曲霉毒素的产生。使用Resinadher和Citrocover时，番茄果实的发病率、菌丝生长和黄曲霉毒素产生量最低，相应值分别为0%、0.0厘米和0.95 ppb，以及7%、0.2厘米和1.77 ppb。使用Citrocover和Resinadher可能是减少番茄果实中黄曲霉生长的可行替代方法。
