Hsieh Shu-Ling, Shih Yi-Wen, Chiu Ying-Ming, Tseng Shao-Feng, Li Chien-Chun, Wu Chih-Chung
Department of Seafood Science, National Kaohsiung University of Science and Technology, Kaohsiung 81157, Taiwan.
Department of Food and Nutrition, Providence University, Taichung 43301, Taiwan.
Plants (Basel). 2021 Nov 25;10(12):2579. doi: 10.3390/plants10122579.
To assess the potential of by-products of the black bean fermented soybean sauce manufacturing process as new functional food materials, we prepared black bean steamed liquid lyophilized product (BBSLP) and analysed its antioxidant effects in vitro. RAW264.7 macrophages were cultured and treated with BBSLP for 24 h, and 1 μg/mL lipopolysaccharide (LPS) was then used for another 24 h to induce inflammation. The cellular antioxidant capacity and inflammatory response were then analysed. Activation of nuclear factor kappa B (NF-κB) signaling in RAW264.7 macrophages was also analysed. Results showed BBSLP had 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium (ABTS) radical-scavenging abilities and reducing power in vitro. The levels of both reactive oxygen species (ROS) and thiobarbituric acid reactive substances (TBARS) were reduced after RAW264.7 macrophages were treated with BBSLP after LPS induction. After RAW264.7 macrophage treatment with BBSLP and induction by LPS, the levels of inflammatory molecules, including nitric oxide (NO), prostaglandin E (PGE), IL-1α, IL-6 and TNF-α, decreased. NF-κB signaling activity was inhibited by reductions in IκB phosphorylation and NF-κB DNA-binding activity after RAW264.7 macrophages were treated with BBSLP after LPS induction. In conclusion, BBSLP has antioxidant and anti-inflammatory capabilities and can be a supplement material for functional food.
为了评估黑豆发酵酱油生产过程中的副产品作为新型功能性食品原料的潜力，我们制备了黑豆蒸煮液冻干产品（BBSLP），并分析了其体外抗氧化作用。培养RAW264.7巨噬细胞，用BBSLP处理24小时，然后用1μg/mL脂多糖（LPS）再处理24小时以诱导炎症。随后分析细胞抗氧化能力和炎症反应。还分析了RAW264.7巨噬细胞中核因子κB（NF-κB）信号的激活情况。结果表明，BBSLP在体外具有2,2-二苯基-1-（2,4,6-三硝基苯基）肼（DPPH）和2,2'-偶氮双（3-乙基苯并噻唑啉-6-磺酸）二铵（ABTS）自由基清除能力和还原能力。LPS诱导后，用BBSLP处理RAW264.7巨噬细胞后，活性氧（ROS）和硫代巴比妥酸反应性物质（TBARS）的水平均降低。用BBSLP处理RAW264.7巨噬细胞并经LPS诱导后，包括一氧化氮（NO）、前列腺素E（PGE）、IL-1α、IL-6和TNF-α在内的炎症分子水平降低。LPS诱导后，用BBSLP处理RAW264.7巨噬细胞后，IκB磷酸化和NF-κB DNA结合活性降低，从而抑制了NF-κB信号活性。总之，BBSLP具有抗氧化和抗炎能力，可作为功能性食品的补充材料。
