Ohishi Natsumi, Hoshika Nanami, Takeda Mizuho, Shibata Kyomi, Yamane Hisakazu, Yokota Takao, Asahina Masashi
Graduate School of Science and Engineering, Teikyo University, 1-1 Toyosatodai, Utsunomiya 320-8551, Tochigi, Japan.
Department of Biosciences, Teikyo University, 1-1 Toyosatodai, Utsunomiya 320-8551, Tochigi, Japan.
Plants (Basel). 2021 Dec 9;10(12):2709. doi: 10.3390/plants10122709.
The spores of , cultured in the dark, form a filamentous structure called protonema. Earlier studies have shown that gibberellin (GA) induces protonema elongation, along with antheridium formation, on the protonema. In this study, we have performed detailed morphological analyses to investigate the roles of multiple phytohormones in antheridium formation, protonema elongation, and prothallus formation in . GA methyl ester is a potent GA that stimulates both protonema elongation and antheridium formation. We found that these effects were inhibited by simultaneous application of abscisic acid (ABA). On the other hand, IAA (indole-3-acetic acid) promoted protonema elongation but reduced antheridium formation, while these effects were partially recovered by transferring to an IAA-free medium. An auxin biosynthesis inhibitor, PPBo (4-phenoxyphenylboronic acid), and a transport inhibitor, TIBA (2,3,5-triiodobenzoic acid), both inhibited protonema elongation and antheridium formation. prothalli are induced from germinating spores under continuous white light. Such development was negatively affected by PPBo, which induced smaller-sized prothalli, and TIBA, which induced aberrantly shaped prothalli. The evidence suggests that the crosstalk between these plant hormones might regulate protonema elongation and antheridium formation in . Furthermore, the possible involvement of auxin in the prothalli development of is suggested.
在黑暗中培养的[某种植物名称]的孢子形成一种称为原丝体的丝状结构。早期研究表明，赤霉素（GA）可诱导原丝体伸长，并在原丝体上形成雄器。在本研究中，我们进行了详细的形态学分析，以研究多种植物激素在[某种植物名称]雄器形成、原丝体伸长和原叶体形成中的作用。GA甲酯是一种有效的GA，可刺激原丝体伸长和雄器形成。我们发现，同时施用脱落酸（ABA）可抑制这些作用。另一方面，吲哚-3-乙酸（IAA）促进原丝体伸长，但减少雄器形成，而将其转移至不含IAA的培养基中后，这些作用可部分恢复。生长素生物合成抑制剂4-苯氧基苯硼酸（PPBo）和运输抑制剂2,3,5-三碘苯甲酸（TIBA）均抑制原丝体伸长和雄器形成。在连续白光下，从萌发的孢子诱导形成[某种植物名称]的原叶体。这种发育受到PPBo（诱导形成较小尺寸的原叶体）和TIBA（诱导形成形状异常的原叶体）的负面影响。证据表明，这些植物激素之间的相互作用可能调节[某种植物名称]的原丝体伸长和雄器形成。此外，提示生长素可能参与了[某种植物名称]原叶体的发育。
