Andonova Tsvetelina, Muhovski Yordan, Fidan Hafize, Slavov Iliya, Stoyanova Albena, Dimitrova-Dyulgerova Ivanka
Department of Botany and Methods of Biology Teaching, Faculty of Biology, University of Plovdiv, Paisii Hilendarski, 4000 Plovdiv, Bulgaria.
Life Sciences Department, Walloon Agricultural Research Centre, 5030 Gembloux, Belgium.
Plants (Basel). 2021 Dec 10;10(12):2715. doi: 10.3390/plants10122715.
Laxm. is used in traditional medicine and has various established biological activities, however, the species is considered to be a potentially invasive alien tree species for Bulgarian flora. However, there is still much to be studied about the phytochemical and biological characteristics of the species. The present study aimed to determine the chemical composition of the ethanol extracts of aerial plant parts, by GC-MS analysis, and to thereby evaluate their in vitro antitumor and antibacterial properties. All three extracts were tested against the HT-29 and PC3 tumor cell lines using the MTT assay. Fifty-six components were identified from leaf, flower, and stem bark extracts, and over 10% were the following constituents: pyrogallol, -terpinyl acetate, neryl acetate, and -terpinyl isobutanoate. The oxygenated monoterpenes predominated in the extracts, followed by the oxygenated aliphatics and phenylpropanoids. Significant antiproliferative activity on the HT-29 cell line (IC-21.44 µg/mL and 23.63 µg/mL, respectively) was found for the flower and leaf extracts. Antibacterial activity was established for the following bacteria strains: ATCC 6633, NCTC 10320, ATCC 8739, ATCC 6027, and ATCC 6380. The stem bark and flower extracts showed better antimicrobial potential. could be considered as a potential source of biologically active substances with antitumor and antibacterial properties.
拉克姆树用于传统医学，具有多种已确定的生物活性，然而，该物种被认为是保加利亚植物群中一种潜在的入侵外来树种。然而，关于该物种的植物化学和生物学特性仍有许多有待研究之处。本研究旨在通过气相色谱 - 质谱分析确定植物地上部分乙醇提取物的化学成分，从而评估其体外抗肿瘤和抗菌特性。使用MTT法对所有三种提取物针对HT - 29和PC3肿瘤细胞系进行了测试。从叶、花和茎皮提取物中鉴定出56种成分，超过10%的成分如下：连苯三酚、α - 萜品醇乙酸酯、橙花醇乙酸酯和α - 萜品醇异丁酸酯。提取物中以氧化单萜类为主，其次是氧化脂肪族和苯丙素类。花和叶提取物对HT - 29细胞系具有显著的抗增殖活性（IC分别为21.44 µg/mL和23.63 µg/mL）。对以下细菌菌株确定了抗菌活性：ATCC 6633、NCTC 10320、ATCC 8739、ATCC 6027和ATCC 6380。茎皮和花提取物显示出更好的抗菌潜力。拉克姆树可被视为具有抗肿瘤和抗菌特性的生物活性物质的潜在来源。
