[肌肉二肽——脂质过氧化的天然抑制剂]

[Muscle dipeptides--natural inhibitors of lipid peroxidation].

作者信息

Dupin A M, Bemanandzara M, Stvolinskiĭ S L, Boldyrev A A, Severin S E

出版信息

Biokhimiia. 1987 May;52(5):782-7.

Abstract

The effects of carnosine (beta-alanyl-L-histidine) and anserine (beta-alanyl-1-methyl-L-histidine) on ascorbate-dependent lipid peroxidation in frog skeletal muscle sarcoplasmic reticulum were studied. It was found that the dipeptides (10-50 mM) cause a 25-90% inhibition of ascorbate-dependent lipid peroxidation and decrease the reaction rate and the amount of end products. The nature of lipid peroxidation primary products in the presence of the dipeptides changes which can be evidenced from changes in their spectral properties. Unlike other known natural antioxidants, skeletal muscle dipeptides do not only inhibit lipid peroxidation but also decrease the level of accumulated lipid peroxidation products. Histidine and beta-alanine, similar to imidazole, glycyl-glycine, arginyl-phenyl alanine and alpha-alanyl-D-histidine do not inhibit lipid peroxidation. At the same time, the carnosine stereoisomer D-carnosine which does not exist in nature exhibits a far greater inhibiting effect as compared to its natural counterpart. It is assumed that the skeletal muscle dipeptides carnosine and anserine are highly effective as natural antioxidants.

摘要

研究了肌肽（β-丙氨酰-L-组氨酸）和鹅肌肽（β-丙氨酰-1-甲基-L-组氨酸）对青蛙骨骼肌肌浆网中抗坏血酸依赖性脂质过氧化的影响。发现二肽（10 - 50 mM）可导致抗坏血酸依赖性脂质过氧化受到25 - 90%的抑制，并降低反应速率和终产物量。二肽存在时脂质过氧化初级产物的性质发生变化，这可从其光谱特性的变化得到证明。与其他已知的天然抗氧化剂不同，骨骼肌二肽不仅抑制脂质过氧化，还降低积累的脂质过氧化产物水平。组氨酸和β-丙氨酸，类似于咪唑、甘氨酰甘氨酸、精氨酰苯丙氨酸和α-丙氨酰-D-组氨酸，不抑制脂质过氧化。同时，自然界不存在的肌肽立体异构体D-肌肽与其天然对应物相比，表现出更大的抑制作用。据推测，骨骼肌二肽肌肽和鹅肌肽作为天然抗氧化剂具有高效性。