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利用脉冲幅度调制荧光法对烟草叶片中病毒感染进行症状前检测。

Pre-Symptomatic Detection of Viral Infection in Tobacco Leaves Using PAM Fluorometry.

作者信息

Grishina Alyona, Sherstneva Oksana, Grinberg Marina, Zdobnova Tatiana, Ageyeva Maria, Khlopkov Andrey, Sukhov Vladimir, Brilkina Anna, Vodeneev Vladimir

机构信息

Department of Biophysics, National Research Lobachevsky State University of Nizhny Novgorod, 23 Gagarin Avenue, 603950 Nizhny Novgorod, Russia.

Department of Biochemistry and Biotechnology, National Research Lobachevsky State University of Nizhny Novgorod, 23 Gagarin Avenue, 603950 Nizhny Novgorod, Russia.

出版信息

Plants (Basel). 2021 Dec 16;10(12):2782. doi: 10.3390/plants10122782.

DOI:10.3390/plants10122782
PMID:34961253
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8707847/
Abstract

Chlorophyll fluorescence imaging was used to study potato virus X (PVX) infection of . Infection-induced changes in chlorophyll fluorescence parameters (quantum yield of photosystem II photochemistry () and non-photochemical fluorescence quenching (NPQ)) in the non-inoculated leaf were recorded and compared with the spatial distribution of the virus detected by the fluorescence of GFP associated with the virus. We determined infection-related changes at different points of the light-induced chlorophyll fluorescence kinetics and at different days after inoculation. A slight change in the light-adapted steady-state values of and NPQ was observed in the infected area of the non-inoculated leaf. In contrast to the steady-state parameters, the dynamics of and NPQ caused by the dark-light transition in healthy and infected areas differed significantly starting from the second day after the detection of the virus in a non-inoculated leaf. The coefficients of correlation between chlorophyll fluorescence parameters and virus localization were 0.67 for and 0.76 for NPQ. In general, the results demonstrate the possibility of reliable pre-symptomatic detection of the spread of a viral infection using chlorophyll fluorescence imaging.

摘要

利用叶绿素荧光成像技术研究了马铃薯X病毒（PVX）的感染情况。记录了未接种叶片中感染诱导的叶绿素荧光参数（光系统II光化学量子产率（）和非光化学荧光猝灭（NPQ））的变化，并将其与通过与病毒相关的绿色荧光蛋白（GFP）荧光检测到的病毒空间分布进行了比较。我们在光诱导叶绿素荧光动力学的不同时间点以及接种后的不同天数测定了与感染相关的变化。在未接种叶片的感染区域观察到和NPQ的光适应稳态值有轻微变化。与稳态参数相反，从在未接种叶片中检测到病毒后的第二天开始，健康区域和感染区域由暗-光转换引起的和NPQ动态变化存在显著差异。叶绿素荧光参数与病毒定位之间的相关系数对于为0.67，对于NPQ为0.76。总体而言，结果表明利用叶绿素荧光成像技术能够可靠地在症状出现前检测病毒感染的传播情况。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e08c/8707847/068dc497e51f/plants-10-02782-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e08c/8707847/c14540275595/plants-10-02782-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e08c/8707847/8348fee846ee/plants-10-02782-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e08c/8707847/4017383199ff/plants-10-02782-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e08c/8707847/93a4fdbad229/plants-10-02782-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e08c/8707847/df71cab583ca/plants-10-02782-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e08c/8707847/e91b289af1f5/plants-10-02782-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e08c/8707847/48bbc7a05421/plants-10-02782-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e08c/8707847/f3df1fd4c941/plants-10-02782-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e08c/8707847/d207c4154e03/plants-10-02782-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e08c/8707847/068dc497e51f/plants-10-02782-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e08c/8707847/c14540275595/plants-10-02782-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e08c/8707847/8348fee846ee/plants-10-02782-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e08c/8707847/4017383199ff/plants-10-02782-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e08c/8707847/93a4fdbad229/plants-10-02782-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e08c/8707847/df71cab583ca/plants-10-02782-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e08c/8707847/e91b289af1f5/plants-10-02782-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e08c/8707847/48bbc7a05421/plants-10-02782-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e08c/8707847/f3df1fd4c941/plants-10-02782-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e08c/8707847/d207c4154e03/plants-10-02782-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e08c/8707847/068dc497e51f/plants-10-02782-g010.jpg

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