De Rie M A, Kabel P, Sauerwein R W, Van Lier R A, Von dem Borne A E, Melief C J, Miedema F
Eur J Immunol. 1987 Jun;17(6):881-6. doi: 10.1002/eji.1830170623.
We produced and characterized a panel of anti-HLA class II monoclonal antibodies (mAb) to study the inhibition of T cell-dependent B cell differentiation. The newly produced mAb belonged to different isotypes and subclasses, and recognized at least two different nonpolymorphic HLA class II determinants. We found that anti-HLA class II mAb inhibited the pokeweed mitogen (PWM)- and interleukin 2-driven T cell-dependent IgM synthesis, but did not affect the phorbol myristate acetate-, phytohemagglutinin-, anti-T3- or PWM-induced T cell proliferation. Moreover, when the mAb were tested in a monocyte-independent B cell differentiation assay in which T cells are activated by insoluble anti-T3 mAb in the absence of accessory cells, no effect was seen on IgM synthesis. These data indicate that inhibition of T cell-dependent B cell differentiation by anti-HLA class II mAb is caused by interference with an accessory cell function essential for B cell differentiation.
我们制备并鉴定了一组抗 HLA II 类单克隆抗体（mAb），以研究对 T 细胞依赖性 B 细胞分化的抑制作用。新制备的 mAb 属于不同的同种型和亚类，并识别至少两种不同的非多态性 HLA II 类决定簇。我们发现抗 HLA II 类 mAb 抑制商陆有丝分裂原（PWM）和白细胞介素 2 驱动的 T 细胞依赖性 IgM 合成，但不影响佛波酯、植物血凝素、抗 T3 或 PWM 诱导的 T 细胞增殖。此外，当在单核细胞非依赖性 B 细胞分化试验中测试这些 mAb 时，在无辅助细胞的情况下，用不溶性抗 T3 mAb 激活 T 细胞，未观察到对 IgM 合成有影响。这些数据表明，抗 HLA II 类 mAb 对 T 细胞依赖性 B 细胞分化的抑制是由于干扰了 B 细胞分化所必需的辅助细胞功能所致。
