van Lier R, Bloemena E, Brouwer M, Van Heijst J, Weinreich S, Aarden L
Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Amsterdam.
Immunology. 1989 Jul;67(3):333-8.
We produced three murine monoclonal antibodies (mAb) directed against the human T-cell differentiation antigen CD2 (formerly T11) and analysed their epitope specificity by E-rosette inhibition, cross-blocking and proliferation-induction experiments. When added together, two mAb (both IgG1 kappa) were able to induce proliferation in peripheral blood T cells. This proliferation was found to be strictly dependent on the presence of monocytes. The polymorphism of Fc receptors (FcR) expressed on human monocytes for murine IgG1 antibodies, which is readily demonstrable in anti-CD3-induced T-cell activation, was not found in the anti-CD2-driven system. Moreover, mAb directed against the 40,000 MW FcRII were unable to inhibit proliferation induced by the mitogenic anti-CD2 combination. Still, F(ab')2 fragments of the mitogenic anti-CD2 antibody combination could not initiate T-cell mitogenesis, despite their ability to induce a rise in the free intracellular [Ca2+]. The contributions of monocytes and of antibody Fc moieties to T-cell proliferation, induced by combinations of anti-CD2 mAb, will be discussed.
我们制备了三种针对人T细胞分化抗原CD2（以前称为T11）的鼠单克隆抗体（mAb），并通过E花环抑制、交叉阻断和增殖诱导实验分析了它们的表位特异性。当两种mAb（均为IgG1 κ）一起添加时，能够诱导外周血T细胞增殖。发现这种增殖严格依赖于单核细胞的存在。在抗CD3诱导的T细胞活化中很容易证明的人单核细胞上表达的针对鼠IgG1抗体的Fc受体（FcR）多态性，在抗CD2驱动的系统中未发现。此外，针对40,000 MW FcRII的mAb不能抑制有丝分裂原性抗CD2组合诱导的增殖。尽管有丝分裂原性抗CD2抗体组合的F(ab')2片段能够诱导细胞内游离[Ca2+]升高，但仍不能启动T细胞有丝分裂。将讨论单核细胞和抗体Fc部分对抗CD2 mAb组合诱导的T细胞增殖的作用。
