肌醇1,4,5-三磷酸和鸟苷三磷酸对垂体微粒体钙释放的影响。
Effect of inositol 1,4,5-trisphosphate and GTP on calcium release from pituitary microsomes.
作者信息
Kiesel L, Lukács G L, Eberhardt I, Runnebaum B, Spät A
出版信息
FEBS Lett. 1987 Jun 8;217(1):85-8. doi: 10.1016/0014-5793(87)81248-6.
Microsomal vesicles from bovine anterior pituitary accumulate Ca2+ and maintain a steady-state ambient Ca2+ level of 200 nM. IP3 and GTP both induce calcium release from the microsomal vesicles. The effect of IP3 is inhibited by polyethylene glycol (PEG), and the effect of GTP is absolutely dependent on PEG. Half-maximal effect of IP3 (without PEG) is 0.26 micron, the maximal calcium release attaining 7% of the A23187-releasable pool. The same values for GTP (in the presence of PEG) are 80 microM and 10%, respectively. GTP potentiates the effect of IP3. This potentiation is not mediated by protein phosphorylation.
来自牛垂体前叶的微粒体囊泡可积累Ca2+，并维持200 nM的稳态胞内Ca2+水平。IP3和GTP均可诱导微粒体囊泡释放钙。IP3的作用可被聚乙二醇（PEG）抑制，而GTP的作用则绝对依赖于PEG。IP3（无PEG）的半数最大效应为0.26微米，最大钙释放量达到A23187可释放池的7%。GTP（在PEG存在下）的相同值分别为80 microM和10%。GTP可增强IP3的作用。这种增强作用不是由蛋白质磷酸化介导的。
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