Department of Ophthalmology, 117913Affiliated Hospital of North Sichuan Medical College, Nanchong, China.
Department of Ophthalmology & Optometry, 74655North Sichuan Medical College, Nanchong, China.
Eur J Ophthalmol. 2022 Sep;32(5):3050-3057. doi: 10.1177/11206721211070305. Epub 2021 Dec 28.
The retina is a highly energy-consuming tissue associated with visual development, and the reduced quality of retinal imaging can be related to myopia. Synthesis of cytochrome c oxidase 1 () and synthesis of cytochrome c oxidase 2 () are involved in ATP (adenosine triphosphate) synthesis and energy metabolism. This study aimed to observe the morphologic changes and investigate the expression of and induced by form-deprivation myopia (FDM) in the retina and sclera of guinea pigs.
Thirty-six 3-week-old male guinea pigs were randomly assigned to one of two groups: (1) the model group (n = 18), in which the right eyes were covered by a thin opaque balloon as FDM group, and the left eyes were uncovered and served as the contralateral control group; (2) the blank control group (n = 18), in which bilateral eye received no manipulation. Eyeballs were enucleated for histological analysis. The retina and sclera of the guinea pigs were separated to determine the protein and mRNA expression levels of and , respectively.
After four weeks of form deprivation (FD), the refractive degree and axial length increased significantly ( < 0.001). The retinal and scleral tissues were moderately thinner, and the ganglion cells and the cells of inner and outer nuclear layers in the retina became fewer. Compared with the contralateral control group ( < 0.001) and the blank control group ( < 0.001), the collagen content of the sclera became less in the FDM group. The protein and mRNA expression levels of and in the FDM group were significantly lower than those in the contralateral control group and the blank control group ( < 0.05).
The morphologies of the retina and sclera were changed, and the expression of and at the protein and transcription levels was significantly reduced in the FDM group. Given these changes, and genes may be involved in myopic progression.
视网膜是一种能量消耗很高的组织，与视觉发育有关，而视网膜成像质量的降低可能与近视有关。细胞色素 c 氧化酶 1 () 和细胞色素 c 氧化酶 2 () 的合成参与了 ATP (三磷酸腺苷) 的合成和能量代谢。本研究旨在观察形态变化，并研究形觉剥夺性近视 (FDM) 诱导的视网膜和巩膜中 和 的表达。
36 只 3 周龄雄性豚鼠随机分为两组：(1)模型组（n = 18），右眼用薄的不透明气球遮盖作为 FDM 组，左眼不遮盖作为对侧对照组；(2)空白对照组（n = 18），双眼均不做任何处理。眼球被取出进行组织学分析。分离豚鼠的视网膜和巩膜，分别测定 和 的蛋白和 mRNA 表达水平。
在 4 周的形觉剥夺后（FD），屈光度和眼轴显著增加（ < 0.001）。视网膜和巩膜组织中度变薄，视网膜节细胞和内、外核层细胞减少。与对侧对照组（ < 0.001）和空白对照组（ < 0.001）相比，FDM 组巩膜胶原含量减少。FDM 组 和 的蛋白和 mRNA 表达水平均明显低于对侧对照组和空白对照组（ < 0.05）。
FDM 组视网膜和巩膜形态发生改变，蛋白和转录水平的 和 表达明显降低。鉴于这些变化， 和 基因可能参与近视进展。
