Jiang Bo, Shi Chun-Sheng
Department of Ophthalmolohy, Anhui NO. 2 Provincial People's Hospital, Anhui Province, China.
Arq Bras Oftalmol. 2020 Jun;83(3):190-195. doi: 10.5935/0004-2749.20200034. Epub 2020 Feb 10.
To investigate periostin and collagen I expression during a scleral remodeling in myopic eyes and to determine their role in collagen remodeling of the myopic sclera.
Fifty one-week-old guinea pigs were divided into the control and form-deprivation myopia (FDM) groups. The eyes of animals in the form-deprivation myopia group were covered for 2, 4, and 8 weeks, or were covered for 4 weeks and then uncovered for 2 weeks. The diopters and axial lengths in the eyes in each group of guinea pigs were measured. Immunohistochemistry and reverse transcription polymerase chain reaction were used to detect the relative protein and mRNA expressions of periostin and collagen I in the scleral tissues of guinea pig.
Before masking, guinea pigs in the control and form-deprivation myopia groups were hypermetropic and did not differ significantly (p>0.05). Hypermetropic refraction in the control group gradually decreased. In guinea pigs from the form-deprivation myopia group, the refractive power gradually changed from +2.14 ± 0.33 D to -7.22 ± 0.51 D, and the axial length gradually changed from 5.92 ± 0.37 mm to 8.05 ± 0.34 mm from before until the end of masking. Before covering, no significant difference was observed in the relative collagen I and periostin mRNA and protein expression levels in the sclera of the guinea pig control and form-deprivation myopia groups (p>0.05). The relative collagen I and periostin protein and mRNA expression levels in the sclera of guinea pigs in the form-deprivation myopia group at 2, 4, and 8 weeks, and after covering the eyes for 4 weeks followed by uncovering for 2 weeks, were significantly lower than those in the control group (p<0.05). The collagen I and periostin mRNA expression levels were positively correlated with protein expression levels in the sclera of guinea pigs (protein: r=0.936, p<0.05; mRNA: r=0.909, p<0.05).
Periostin was expressed in the myopic sclera of guinea pigs, and changes in periostin and collagen I expression were highly consistent. Periostin and collagen I may be involved in the regulation of scleral remodeling in myopia.
研究骨膜蛋白和Ⅰ型胶原蛋白在近视眼巩膜重塑过程中的表达情况，并确定它们在近视性巩膜胶原重塑中的作用。
将51只1周龄豚鼠分为对照组和形觉剥夺性近视（FDM）组。对形觉剥夺性近视组动物的眼睛分别遮盖2周、4周和8周，或遮盖4周后再暴露2周。测量每组豚鼠眼睛的屈光度和眼轴长度。采用免疫组织化学和逆转录聚合酶链反应检测豚鼠巩膜组织中骨膜蛋白和Ⅰ型胶原蛋白的相对蛋白和mRNA表达。
遮盖前，对照组和形觉剥夺性近视组豚鼠均为远视，差异无统计学意义（p>0.05）。对照组的远视屈光度逐渐降低。形觉剥夺性近视组豚鼠在遮盖期间，屈光度从+2.14±0.33D逐渐变为-7.22±0.51D，眼轴长度从5.92±0.37mm逐渐变为8.05±0.34mm。遮盖前，豚鼠对照组和形觉剥夺性近视组巩膜中Ⅰ型胶原蛋白和骨膜蛋白的相对mRNA和蛋白表达水平差异无统计学意义（p>0.05）。形觉剥夺性近视组豚鼠在遮盖2周、4周和8周时，以及遮盖4周后再暴露2周时，巩膜中Ⅰ型胶原蛋白和骨膜蛋白的相对mRNA和蛋白表达水平均显著低于对照组（p<0.05）。豚鼠巩膜中Ⅰ型胶原蛋白和骨膜蛋白的mRNA表达水平与蛋白表达水平呈正相关（蛋白：r=0.936，p<0.05；mRNA：r=0.909，p<0.05）。
骨膜蛋白在豚鼠近视性巩膜中表达，且骨膜蛋白和Ⅰ型胶原蛋白的表达变化高度一致。骨膜蛋白和Ⅰ型胶原蛋白可能参与近视性巩膜重塑的调节。
