Eye Center, Tianjin Medical University, Tianjin, 300070, People's Republic of China.
Graefes Arch Clin Exp Ophthalmol. 2010 Apr;248(4):519-25. doi: 10.1007/s00417-009-1287-z. Epub 2010 Feb 5.
By investigating the effects of recombinant human IGF-2 (rhIGF-2) on the diopter, axial eye length and the expression of IGF-2 in non-form-deprivation (FD) and FD eyes of guinea pig, we tried to elucidate the relationship between the effects of rhIGF-2 on eye growth and FD in guinea pig.
Eighty 3-week-old guinea pigs were included in the study, which were divided into two groups randomly. Group A (n = 40) was the non-FD group, Thirty-two guinea pigs received intravitreal injections of either 1 ng, 10 ng, 100 ng rhIGF-2 or bovine serum albumin (BSA) to the right eye; eight guinea pigs who received no intravitreal injections served as control. Group B (n = 40) was the FD group; the right eyes were form-deprived, and received the same disposals as group A. The diopter and the axial eye length of all guinea pigs were measured at day 14. The expression of IGF-2 in the retina was measured by Western blot.
After 14 days, FD eyes were high myopia. The axial length of FD eyes was significant for longer than that of non-FD eyes. The expression of IGF-2 in the retina of FD eyes was up-regulated. In non-FD group, there was no significant difference in the diopter and axial eye length among rhIGF-2 injection eyes, BSA injection eyes and control eyes, but the expression of IGF-2 in the retina of 10 ng and 100 ng rhIGF-2 injection eyes was obviously down-regulated. In the FDM (form-deprivation myopia) group, the myopic diopter and axial eye length increased in 10 ng and 100 ng rhIGF-2 injection eyes compared with those in BSA injection eyes and control eyes. The level of IGF-2 expression in the retina of 10 ng and 100 ng rhIGF-2 injection eyes was obviously down-regulated. The diopter and expression of IGF-2 in the retina of rhIGF-2 injection eyes was negatively correlated with the dose of each injection, which was positively correlated with the axial length.
RhIGF-2 intravitreous injection does not affect the diopter and axial length in non-FD eyes of guinea pig, while it can induce a significant increase in myopic diopter and axial length of FD eyes. RhIGF-2 can promote the development of FDM on the condition of FD.
通过研究重组人生长因子 2（rhIGF-2）对豚鼠非形觉剥夺（FD）和 FD 眼屈光度、眼轴长度和 IGF-2 表达的影响，探讨 rhIGF-2 对豚鼠眼生长和 FD 的影响关系。
将 80 只 3 周龄豚鼠随机分为两组。A 组（n = 40）为非 FD 组，32 只豚鼠右眼分别玻璃体腔注射 1ng、10ng、100ng rhIGF-2 或牛血清白蛋白（BSA）；8 只不做玻璃体腔注射的豚鼠作为对照。B 组（n = 40）为 FD 组；右眼形觉剥夺，并给予与 A 组相同的处理。第 14 天测量所有豚鼠的屈光度和眼轴长度。Western blot 法检测视网膜 IGF-2 的表达。
14 天后，FD 眼呈高度近视。FD 眼眼轴长度明显长于非 FD 眼。FD 眼视网膜 IGF-2 表达上调。非 FD 组，rhIGF-2 注射眼、BSA 注射眼和对照组之间的屈光度和眼轴长度无显著差异，但 10ng 和 100ng rhIGF-2 注射眼视网膜 IGF-2 的表达明显下调。在 FDm（形觉剥夺性近视）组中，与 BSA 注射眼和对照组相比，10ng 和 100ng rhIGF-2 注射眼的近视屈光度和眼轴长度增加，视网膜 IGF-2 的表达水平明显下调。rhIGF-2 注射眼的屈光度和视网膜 IGF-2 的表达与各注射剂量呈负相关，与眼轴长度呈正相关。
rhIGF-2 玻璃体腔注射不影响豚鼠非 FD 眼的屈光度和眼轴长度，而能显著诱导 FD 眼近视屈光度和眼轴长度的增加。rhIGF-2 可在 FD 的条件下促进 FDm 的发生。
