Facultad de Ingenierías y Ciencias Aplicadas, Ingeniería Ambiental, Grupo de Investigación en Biodiversidad, Medio Ambiente y Salud (BIOMAS), Universidad de las Américas, Quito, Ecuador.
Department of Entomology, University of Minnesota, St. Paul, Minnesota, USA.
Genome Biol Evol. 2022 Jan 4;14(1). doi: 10.1093/gbe/evab286.
We sequence, assemble, and annotate the genome of Atopsyche davidsoni Sykora, 1991, the first whole-genome assembly for the caddisfly family Hydrobiosidae. This free-living and predatory caddisfly inhabits streams in the high-elevation Andes and is separated by more than 200 Myr of evolutionary history from the most closely related caddisfly species with genome assemblies available. We demonstrate the promise of PacBio HiFi reads by assembling the most contiguous caddisfly genome assembly to date with a contig N50 of 14 Mb, which is more than 6× more contiguous than the current most contiguous assembly for a caddisfly (Hydropsyche tenuis). We recover 98.8% of insect BUSCO genes indicating a high level of gene completeness. We also provide a genome annotation of 12,232 annotated proteins. This new genome assembly provides an important new resource for studying genomic adaptation of aquatic insects to harsh, high-altitude environments.
我们对戴维森石蛾（Atopsyche davidsoni Sykora, 1991）的基因组进行了测序、组装和注释，这是第一个水石蛾科（Hydrobiosidae）的全基因组组装。这种自由生活和捕食性的石蛾栖息在安第斯山脉高海拔地区的溪流中，与具有基因组组装的最接近的石蛾物种相隔超过 2 亿年的进化历史。我们通过组装迄今为止最连续的石蛾基因组组装，展示了 PacBio HiFi 读数的潜力，该组装的 contig N50 为 14Mb，比当前最连续的石蛾组装（Hydropsyche tenuis）连续度高出 6 倍以上。我们恢复了 98.8%的昆虫 BUSCO 基因，表明基因完整性水平很高。我们还提供了 12,232 个注释蛋白的基因组注释。这个新的基因组组装为研究水生昆虫对恶劣、高海拔环境的基因组适应性提供了一个重要的新资源。
