降低 COVID-19 患者唾液中 SARS-CoV-2 的病毒载量。

Reducing the viral load of SARS-CoV-2 in the saliva of patients with COVID-19.

机构信息

Department of Periodontology, Dental School, Rio de Janeiro State University, Rio de Janeiro, Brazil.

Histocompatibility and Cryopreservation Laboratory, Rio de Janeiro State University, Rio de Janeiro, Brazil.

出版信息

Oral Dis. 2022 Nov;28 Suppl 2:2474-2480. doi: 10.1111/odi.14118. Epub 2022 Jan 6.

DOI:10.1111/odi.14118

PMID:34963033

Abstract

OBJECTIVE

To evaluate the reduction in the salivary viral load using oral antiseptic mouthwashes in patients testing positive for COVID-19.

METHODS

Sixty-three individuals were recruited after testing positive for COVID-19 by real-time RT-PCR assay and divided into 5 groups. Group 1 received sterile water, group 2 received 1.5% hydrogen peroxide solution (HP), group 3 received 0.12% chlorhexidine (CHX), group 4 received 0.1% sodium hypochlorite solution (NaClO), and group 5 received sequential rinses using CHX and HP. After collecting the initial saliva sample, individuals were asked to use the designated mouthwash for 1 min. Additional saliva samples were collected immediately after rinsing, 15, and 30 min after rinsing. Real-time RT-PCR assays for RNA detection of SARS-CoV-2 were performed on the saliva samples.

RESULTS

There were no significant differences among the experimental groups and the control group in any period. Compared to the baseline values, there was a significant reduction in the number of copies of SARS-CoV-2 after 30 min in group 2 and immediately after the initial mouthwash in group 4.

CONCLUSIONS

No experimental group demonstrated a significant reduction in the viral load compared to the control group.

摘要

目的

评估在 COVID-19 检测呈阳性的患者中使用口腔抗菌漱口水对唾液病毒载量的降低作用。

方法

通过实时 RT-PCR 检测，在 63 名 COVID-19 检测呈阳性的个体中招募了受试者，并将其分为 5 组。第 1 组接受无菌水，第 2 组接受 1.5%过氧化氢溶液（HP），第 3 组接受 0.12%洗必泰（CHX），第 4 组接受 0.1%次氯酸钠溶液（NaClO），第 5 组接受 CHX 和 HP 序贯冲洗。在收集初始唾液样本后，要求个体使用指定的漱口水漱口 1 分钟。在漱口后立即、15 分钟和 30 分钟收集额外的唾液样本。对唾液样本进行 SARS-CoV-2 的 RNA 检测实时 RT-PCR 分析。