Division of Pulmonology, Department of Internal Medicine, Institute of Chest Diseases, Severance Hospital, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, 03722, Republic of Korea.
Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Seoul National University Bundang Hospital, 82 Gumi-ro, 173 Beon-gil, Bundang-gu, Seongnam-si, Gyeonggi-do, 463-707, Republic of Korea.
Respir Res. 2021 Dec 28;22(1):322. doi: 10.1186/s12931-021-01919-1.
Lung cancer is the primary cause of cancer-related deaths worldwide. The human lung serves as a niche to a unique and dynamic bacterial community that is related to the development of multiple diseases. Here, we investigated the differences in the lung microbiomes of patients with lung cancer.
16S rRNA sequencing was performed to evaluate the respiratory tract microbiome present in the bronchoalveolar lavage fluid. Patients were stratified based on programmed death-ligand 1 (PD-L1) expression levels and immunotherapy responses.
In total, 84 patients were prospectively analyzed, of which 59 showed low (< 10%), and 25 showed high (≥ 10%) PD-L1 expression levels. The alpha and beta diversities did not significantly differ between the two groups. Veillonella dispar was dominant in the high-PD-L1 group; the population of Neisseria was significantly higher in the low-PD-L1 group than in the high-PD-L1 group. In the immunotherapy responder group, V. dispar was dominant, while Haemophilus influenzae and Neisseria perflava were dominant in the non-responder group.
The abundances of Neisseria and V. dispar differed significantly in relation to PD-L1 expression levels and immunotherapy responses.
肺癌是全球癌症相关死亡的主要原因。人类肺部是一个独特而动态的细菌群落的栖息地，与多种疾病的发展有关。在这里，我们研究了肺癌患者肺部微生物组的差异。
使用 16S rRNA 测序来评估支气管肺泡灌洗液中的呼吸道微生物组。根据程序性死亡配体 1（PD-L1）表达水平和免疫治疗反应对患者进行分层。
总共前瞻性分析了 84 名患者，其中 59 名患者的 PD-L1 表达水平较低（<10%），25 名患者的 PD-L1 表达水平较高（≥10%）。两组之间的 alpha 和 beta 多样性没有显著差异。高 PD-L1 组中优势菌为迪斯帕 Veillonella；低 PD-L1 组中奈瑟氏菌的丰度明显高于高 PD-L1 组。在免疫治疗应答组中，迪斯帕 Veillonella 占优势，而流感嗜血杆菌和奈瑟氏佩弗拉瓦 Neisseria perflava 则在无应答组中占优势。
奈瑟氏菌和迪斯帕 Veillonella 的丰度与 PD-L1 表达水平和免疫治疗反应显著相关。
