The Key Laboratory of Eco-Environment in Three Gorges Reservoir Region, Chongqing University, Chongqing 400045, PR China; College of Environment and Ecology, Chongqing University, Chongqing 400045, PR China.
College of Environment and Ecology, Chongqing University, Chongqing 400045, PR China.
Sci Total Environ. 2022 Mar 25;814:152635. doi: 10.1016/j.scitotenv.2021.152635. Epub 2021 Dec 25.
At present, the improvement of nitrate and mixed heavy metals removal in wastewater by microorganism are urgently needed. Previous studies have shown that Pseudomonas hibiscicola strain L1 exhibited Ni(II) removal ability under aerobic denitrification. In this study, the characteristics of the free strain L1, peanut shell biochar (PBC) and further the co-system of strain L1 immobilized on PBC were investigated for the removal of Ni(II), Cr(VI), Cu(II) and nitrate in mix-wastewater. The results illustrated that strain L1 could remove 15.51% - 32.55% of Ni(II) (20-100 mg·L), and removal ratios by co-system were ranked as Ni(II) (81.17%) > Cu(II) (45.84%) > Cr(VI) (38.21%). Scanning Electron Microscope (SEM), X-ray Diffractometer (XRD) and Fourier Transform Infrared Spectroscopy (FTIR) images indicated that the strain L1 immobilized well on PBC and had vigorous biological activity; the crystals of Ni(OH), Cu(OH) and CrO(OH) etc. were formed on surface of co-system with various functional groups participated in. In Sequential Batch Reactor (SBR), the pollutant removal ratios by co-system were higher than that by free strain L1. This study illustrated that the co-system of strain L1 immobilized on PBC was qualified to be applied for practical scenarios of effective heavy metal removal of electroplating mix-wastewater.
目前，迫切需要通过微生物提高废水中硝酸盐和混合重金属的去除率。先前的研究表明，假单胞菌 Hibiscicola 菌株 L1 在好氧反硝化条件下具有去除 Ni(II)的能力。在这项研究中，考察了游离菌株 L1、花生壳生物炭（PBC）以及进一步固定在 PBC 上的菌株 L1 共系统对混合废水中 Ni(II)、Cr(VI)、Cu(II)和硝酸盐的去除特性。结果表明，菌株 L1 可以去除 15.51%-32.55%的 Ni(II)（20-100mg·L），共系统的去除率依次为 Ni(II)（81.17%）>Cu(II)（45.84%）>Cr(VI)（38.21%）。扫描电子显微镜（SEM）、X 射线衍射仪（XRD）和傅里叶变换红外光谱（FTIR）图像表明，菌株 L1 很好地固定在 PBC 上，具有很强的生物活性；在共系统表面形成了 Ni(OH)、Cu(OH)和 CrO(OH)等晶体，多种官能团参与其中。在序批式反应器（SBR）中，共系统的污染物去除率高于游离菌株 L1。本研究表明，固定在 PBC 上的菌株 L1 共系统有资格应用于电镀混合废水有效去除重金属的实际情况。
