Bazin R, Lemieux R
J Immunol. 1987 Aug 1;139(3):780-7.
It has been shown recently that monocyte-macrophage cells produce a growth factor (HGF) active on newly formed B cell hybridomas. We have studied the effect of HGF on the proliferation of an HGF-sensitive clone of B cell hybridoma. Results obtained showed that the murine P388D1 cell-derived HGF has a m.w. of 29,000 and an isoelectric point (pI) of 6.2 whereas the human monocyte-derived HGF has a m.w. of 34,000 and a pI of 4.9. The HGF activity was not mediated by interleukin 1 because the two activities could be completely separated by gel filtration. Results obtained in in vivo experiments showed that HGF-sensitive cells are tumorigenic in mice. The finding that the HGF biochemical parameters (m.w. and pI) are similar to the ones of a recently described plasmacytoma growth factor suggests that HGF and the plasmacytoma growth factor are similar and that the HGF sensitivity of SP 2/O myeloma cells is reactivated after fusion with normal B lymphocytes.
最近研究表明，单核细胞 - 巨噬细胞可产生一种对新形成的B细胞杂交瘤有活性的生长因子（HGF）。我们研究了HGF对B细胞杂交瘤的HGF敏感克隆增殖的影响。结果显示，鼠源P388D1细胞产生的HGF分子量为29,000，等电点（pI）为6.2，而人单核细胞产生的HGF分子量为34,000，pI为4.9。HGF活性不是由白细胞介素1介导的，因为这两种活性可通过凝胶过滤完全分离。体内实验结果表明，HGF敏感细胞在小鼠体内具有致瘤性。HGF的生化参数（分子量和pI）与最近描述的浆细胞瘤生长因子相似，这一发现表明HGF与浆细胞瘤生长因子相似，并且SP 2/O骨髓瘤细胞与正常B淋巴细胞融合后，其对HGF的敏感性会重新激活。
