Bazin R, Lemieux R
Canadian Red Cross Blood Transfusion Service, Terminus, Québec.
J Immunol Methods. 1989 Jan 17;116(2):245-9. doi: 10.1016/0022-1759(89)90210-x.
The addition of macrophage feeder cells or conditioned medium has been shown to increase the yield of murine hybridomas obtained after the fusion of myeloma cells and activated B lymphocytes. It has been shown recently that the conditioned medium contains a growth factor (HGF) active on newly formed hybridomas and that the human HGF is similar to B cell stimulatory factor 2 which can induce the synthesis of antibodies in transformed B cells. We have compared in several fusion experiments the stimulatory effects of HGF both on the yield of hybridomas and on the number of antibody-secreting hybridomas. The results obtained clearly showed that while the stimulatory effect of HGF on the yield of growth-positive wells was variable and sometimes barely detectable, the proportion of growth-positive wells containing monoclonal antibodies was consistently much higher in the HGF-containing cultures. These results suggest that the majority of the antibody-secreting newly formed hybridomas are sensitive to HGF and indicate that HGF is a very useful culture supplement for the generation of a high number of antibody-producing hybridomas even if it may not increase significantly the yield of viable hybridomas.
已证明添加巨噬细胞饲养细胞或条件培养基可提高骨髓瘤细胞与活化B淋巴细胞融合后获得的鼠杂交瘤的产量。最近已表明，条件培养基含有对新形成的杂交瘤有活性的生长因子（HGF），并且人HGF类似于可诱导转化B细胞中抗体合成的B细胞刺激因子2。我们在多个融合实验中比较了HGF对杂交瘤产量和分泌抗体的杂交瘤数量的刺激作用。获得的结果清楚地表明，虽然HGF对生长阳性孔产量的刺激作用是可变的，有时几乎检测不到，但在含HGF的培养物中，含有单克隆抗体的生长阳性孔的比例一直要高得多。这些结果表明，大多数新形成的分泌抗体的杂交瘤对HGF敏感，并表明HGF是用于产生大量产生抗体的杂交瘤的非常有用的培养补充剂，即使它可能不会显著提高活杂交瘤的产量。
