Webb A C, Collins K L, Snyder S E, Alexander S J, Rosenwasser L J, Eddy R L, Shows T B, Auron P E
J Exp Med. 1987 Jul 1;166(1):77-94. doi: 10.1084/jem.166.1.77.
An LPS-stimulated, human monocyte cDNA library was screened for stimulation-specific clones. One clone (pcD-1214) contained a 1.9-kb pair insert that hybridized to a 2,000-nucleotide mRNA expressed by peripheral blood monocytes, the histiocytic lymphoma cell line U937, and umbilical cord endothelial cells. The 415-amino-acid precursor polypeptide predicted from the cDNA (46,596 molecular weight) has a putative 22-residue signal peptide and approximately 35% homology with members of the serine protease inhibitor (Serpin) superfamily. On the basis of amino acid homology and alignment of COOH-terminal residues within the Serpin-reactive center, the clone pcD-1214 was identified as coding for an Arg-Serpin. Southern blot analysis of human-mouse somatic cell hybrid DNA locates the Arg-Serpin gene on human chromosome 18. A perfect match between amino acid residues 347-376 in this Arg-Serpin and the published sequence of a 30-residue, tryptic peptide from the COOH-terminus of a monocyte plasminogen activator-inhibitor (PAI-2), strongly suggests that the Arg-Serpin encoded by pcD-1214 is PAI-2.
利用脂多糖刺激的人单核细胞cDNA文库筛选刺激特异性克隆。一个克隆（pcD - 1214）含有一个1.9千碱基对的插入片段，该片段与外周血单核细胞、组织细胞淋巴瘤细胞系U937和脐带内皮细胞表达的2000个核苷酸的mRNA杂交。从该cDNA预测的415个氨基酸的前体多肽（分子量46,596）具有一个推定的22个残基的信号肽，并且与丝氨酸蛋白酶抑制剂（Serpin）超家族成员具有约35%的同源性。基于氨基酸同源性以及Serpin反应中心内COOH末端残基的比对，克隆pcD - 1214被鉴定为编码一种精氨酸 - Serpin。对人 - 鼠体细胞杂交DNA进行Southern印迹分析，将精氨酸 - Serpin基因定位在人类18号染色体上。该精氨酸 - Serpin中347 - 376位氨基酸残基与单核细胞纤溶酶原激活物抑制剂（PAI - 2）COOH末端的一个30个残基的胰蛋白酶肽的已发表序列完全匹配，这强烈表明pcD - 1214编码的精氨酸 - Serpin就是PAI - 2。
