National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, P. R. China.
Jiangxi Health Vocational College, Nanchang 330052, China.
Biomacromolecules. 2022 Jan 10;23(1):409-423. doi: 10.1021/acs.biomac.1c01360. Epub 2021 Dec 29.
The purpose of this study was to construct a glycogen (Gly)-based nanoparticle (NP) with liver-targeted and redox response to effectively deliver resveratrol (Res) for improving nonalcoholic fatty liver disease (NAFLD). Herein, Gly was modified using α-lipoic acid (α-LA) and lactobionic acid (Lac) to obtain an amphiphilic polymer (Gly-LA-Lac), which was self-assembled in water and then encapsulated in Res to form Res NPs with excellent stability. As expected, the Res NPs exhibited liver-targeted and redox response release behavior. cell studies demonstrated that the nanocarrier treatment enhanced the cellular uptake of Res and reduced oxidative stress and inflammatory factor levels. Meanwhile, the tests proved that the nanocarriers effectively reduced hepatic lipid accumulation and oxidative stress levels regulating the TLR4/NF-κB signal pathway to improve liver damage in NAFLD mice. In conclusion, this study provides a promising strategy through the construction of Gly-based nanocarriers for the encapsulation of Res to effectively alleviate the process of NAFLD.
本研究旨在构建一种基于糖原（Gly）的纳米粒子（NP），具有肝靶向和氧化还原响应性，以有效递送白藜芦醇（Res），从而改善非酒精性脂肪性肝病（NAFLD）。在此，使用α-硫辛酸（α-LA）和乳酰乳酸（Lac）对 Gly 进行修饰，得到一种两亲性聚合物（Gly-LA-Lac），它在水中自组装，然后将 Res 包裹在其中，形成具有优异稳定性的 Res NPs。正如预期的那样，Res NPs 表现出肝靶向和氧化还原响应性释放行为。细胞研究表明，该纳米载体处理增强了 Res 的细胞摄取，并降低了氧化应激和炎症因子水平。同时，试验证明纳米载体有效减少了肝脂质积累和氧化应激水平，通过调节 TLR4/NF-κB 信号通路，改善了 NAFLD 小鼠的肝损伤。总之，本研究通过构建基于 Gly 的纳米载体来封装 Res，为有效缓解 NAFLD 提供了一种有前途的策略。
