红海软珊瑚的细胞毒性与化学剖析

Cytotoxicity and chemical profiling of the Red Sea soft corals .

作者信息

Mahmoud Amany Hamouda, Zidan Sabry A H, Samy Mamdouh Nabil, Alian Abdallah, Abdelmohsen Usama Ramadan, Fouad Mostafa Ahmed, Kamel Mohamed Salah, Matsunami Katsuyoshi

机构信息

Department of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia, Egypt.

Department of Pharmacognosy, Faculty of Pharmacy, Al-Azhar University, Assiut-Branch, Assiut, Egypt.

出版信息

Nat Prod Res. 2022 Aug;36(16):4261-4265. doi: 10.1080/14786419.2021.1974437. Epub 2021 Dec 29.

DOI:10.1080/14786419.2021.1974437

PMID:34965809

Abstract

The objective of this research was to evaluate the cytotoxic activities of the fractions and isolated compounds of the soft corals against A549, MCF-7 and HepG2 cell lines by MTT assay method, and to chemically investigate the various metabolites of its total extract using LC-HR-ESI-MS metabolomic profiling. The metabolomic profiling revealed the presence of various metabolites, mainly sesquiterpenes and steroids reported for the first time in Additionally, eight compounds (-) have been isolated from the -hexane-chloroform (1:1) fraction that exhibited noticeable activity towards A549, MCF-7 and HepG2 cell lines. The steroids ( and ), and the sesquiterpene () exerted noticeable activity against A549 cell line (IC 28.5 ± 4.4, 36.9 ± 2.9 and 67.3 ± 9.9 µM/mL, respectively) compared to etoposide as standard cytotoxic agent (IC 48.3 ± 7.6 µM/mL). Compound also exhibited cytotoxicity against MCF-7 cell line (IC 55.3 ± 4.9 µM/mL).

摘要

本研究的目的是通过MTT法评估软珊瑚的各馏分和分离化合物对A549、MCF-7和HepG2细胞系的细胞毒性活性，并使用LC-HR-ESI-MS代谢组学分析方法对其总提取物的各种代谢产物进行化学研究。代谢组学分析揭示了各种代谢产物的存在，主要是首次报道的倍半萜和甾体。此外，已从正己烷-氯仿（1:1）馏分中分离出8种化合物（-），它们对A549、MCF-7和HepG2细胞系表现出显著活性。与作为标准细胞毒性剂的依托泊苷（IC50 48.3±7.6μM/mL）相比，甾体（和）以及倍半萜（）对A549细胞系表现出显著活性（IC50分别为28.5±4.4、36.9±2.9和67.3±9.9μM/mL）。化合物对MCF-7细胞系也表现出细胞毒性（IC50 55.3±4.9μM/mL）。