Askari-Zahabi Khadijeh, Abbasnejad Mehdi, Kooshki Razieh, Raoof Maryam, Esmaeili-Mahani Saeed, Pourrahimi Ali Mohammad, Zamyad Mahnaz
Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran.
Department of Biology, Faculty of Sciences, Lorestan University, Khorramabad, Iran.
Korean J Pain. 2022 Jan 1;35(1):22-32. doi: 10.3344/kjp.2022.35.1.22.
Migraine headaches have been associated with sensory hyperactivity and anomalies in social/emotional responses. The main objective of this study was to evaluate the potential involvement of orexin 1 receptors (Orx1R) within the basolateral amygdala (BLA) in the modulation of pain and psychosocial dysfunction in a nitroglycerin (NTG)-induced rat model of migraine.
Adult male Wistar rats were injected with NTG (5 mg/kg, intraperitoneal) every second day over nine days to induce migraine. The experiments were done in the following six groups (6 rats per group): untreated control, NTG, NTG plus vehicle, and NTG groups that were post-treated with intra-BLA microinjection of Orx1R antagonist SB-334867 (10, 20, and 40 nM). Thermal hyperalgesia was assessed using the hot plate and tail-flick tests. Moreover, the elevated plus maze (EPM) and open field (OF) tests were used to assess anxiety-like behaviors. The animals' sociability was evaluated using the three-chamber social task. The NTG-induced photophobia was assessed using a light-dark box.
We observed no change in NTG-induced thermal hyperalgesia following administration of SB-334867 (10, 20, and 40 nM). However, SB-334867 (20 and 40 nM) aggravated the NTG-induced anxiogenic responses in both the EPM and OF tasks. The NTG-induced social impairment was overpowered by SB-334867 at all doses. Time spent in the dark chamber of light-dark box was significantly increased in rats treated with SB-334867 (20 and 40 nM/rat).
The findings suggest a role for Orx1R within the BLA in control comorbid affective complaints with migraine in rats.
偏头痛与感觉过敏以及社交/情绪反应异常有关。本研究的主要目的是评估基底外侧杏仁杏仁杏仁核杏仁核（BLA）内的食欲素1受体（Orx1R）在硝酸甘油（NTG）诱导的偏头痛大鼠模型中对疼痛和心理社会功能障碍调节的潜在作用。
成年雄性Wistar大鼠每隔一天腹腔注射NTG（5毫克/千克），共注射九天以诱导偏头痛。实验分为以下六组（每组6只大鼠）：未处理的对照组、NTG组、NTG加溶剂组，以及经BLA内微量注射Orx1R拮抗剂SB - 334867（10、20和40纳摩尔）后处理的NTG组。使用热板法和甩尾试验评估热痛觉过敏。此外，使用高架十字迷宫（EPM）和旷场试验（OF）评估焦虑样行为。使用三室社交任务评估动物的社交能力。使用明暗箱评估NTG诱导的畏光反应。
给予SB - 334867（10、20和40纳摩尔）后，我们观察到NTG诱导的热痛觉过敏没有变化。然而，SB - 334867（20和40纳摩尔）在EPM和OF任务中加重了NTG诱导的焦虑反应。在所有剂量下，SB - 334867均克服了NTG诱导的社交障碍。用SB - 334867（20和40纳摩尔/只大鼠）处理的大鼠在明暗箱暗室中停留的时间显著增加。
研究结果表明，BLA内的Orx1R在控制大鼠偏头痛合并的情感障碍中发挥作用。
