Tsai T H, Scheving L E, Marques N, Sánchez de la Peña S, Halberg F
Prog Clin Biol Res. 1987;227A:193-8.
An infradian modulation with a 5-day period characterizes corneal mitoses in adult female inbred Lewis rats. The animals had been standardized, two per cage, with Purina Chow and deionized water freely available at 24 +/- 1 degree C and approximately 50% relative humidity, on six different sequences of light (L) alternating every 12 hr with darkness (D). The LD 12:12 regimen was staggered by approximately 4hr in the six environments to approximate, by sampling within 1 hr, sampling over a 24-hr LD span. Bedding was changed 3 days before each day of killing. During each of 8 consecutive days, one rat from each environment was anesthetized with ether and the eyeballs were removed. Mitotic indices, computed separately for each eye, were analyzed by linear and nonlinear least-squares analyses. For a prominent circadian component of mitotic activity, the 95% confidence interval (CI) extended from 23.84 to 24.85 hr, with a point estimate of 24.32 hr; for a second component of 117 hr, the Cl extended from 90 to 163 hr. Whether the approximate 5-day infradian rhythm is of ovarian or other origin is a problem for further study.
成年雌性近交系Lewis大鼠角膜有丝分裂具有以5天为周期的亚日节律调节。这些动物已标准化饲养，每笼两只，自由提供普瑞纳饲料和去离子水，饲养温度为24±1℃，相对湿度约为50%，处于六种不同的光照（L）和黑暗（D）交替序列中，每12小时交替一次。在六个环境中，12:12的光照-黑暗方案错开约4小时，以便在1小时内取样，从而在24小时的光照-黑暗周期内进行取样。在每次处死前3天更换垫料。在连续8天中的每一天，从每个环境中取出一只大鼠，用乙醚麻醉后摘除眼球。分别计算每只眼睛的有丝分裂指数，并通过线性和非线性最小二乘法分析。对于有丝分裂活动的一个显著的昼夜节律成分，95%置信区间（CI）为23.84至24.85小时，点估计值为24.32小时；对于117小时的第二个成分，CI为90至163小时。大约5天的亚日节律是源于卵巢还是其他原因，是有待进一步研究的问题。
