Carandente F, Angeli A, Crosignani P, Dammacco F, De Cecco L, Ferrario V F, Halberg F, Marrama P, Martini L, Massobrio M
Prog Clin Biol Res. 1987;227B:533-48.
Rectal temperatures were measured automatically every 10 min for part or most of two menstrual cycles in ten clinically healthy young women, 20-30 years of age, with a wearable instrument, the Polychronor. Occasional malfunction of the instrumentation resulted in corresponding gaps in the series. Data were examined by chronograms, plexograms, and chronobiologic serial sections computed with the fit of a 24-hr period, population-mean cosinor, and linear-nonlinear least-squares analyses. Single cosinor-derived circadian parameters were next fitted with a cosine curve of a period equal to the number of days of the corresponding intermenstruum. Second-order infradian inferential statistics were calculated next; the first day of menstruation was used as acrophase reference. A population-mean cosinor at the intermenstrual period yields a temperature acrophase of -279 degrees, with the 95% confidence interval extending from -254 degrees to -312 degrees. Since the intermenstruum differs in different subjects and/or in different menstrual cycles of a given woman, this acrophase corresponds to different time intervals from the first day of menstruation in different cases. This acrophase thus indicates the relative timing within the menstrual cycle of overall high rectal temperatures. On four subjects in four stages of their menstrual cycle, plasma was also obtained at 2-hr intervals around the clock. Ten hormones were determined. The sparse endocrine sampling along the menstrual cycle notwithstanding, a circatrigintan rhythm in all hormones investigated was demonstrated for a woman 26 years of age. At the period corresponding to the intermenstrual interval, the acrophases for T3, cortisol, FSH, testosterone, DHEA-S, T4, and LH occurred before the circatrigintan rectal temperature acrophase, whereas the acrophases for prolactin, estradiol, and progesterone occurred near or shortly after the rectal temperature acrophase. Whereas earlier circatrigintan mapping of adult women had been summarized on a group basis, this study allows individualized circatrigintan rhythm assessment. Circatrigintan, like circadian and circannual, acrophase and amplitude relations do not necessarily imply causal relations, yet they are an indispensable quantitative reference standard for the study of basic mechanisms and for diagnosis and intervention, including endeavors in planned parenthood that might take into account the organism's dynamics with multiple frequencies.
使用可穿戴仪器Polychronor，对10名年龄在20至30岁、临床健康的年轻女性的部分或大部分两个月经周期，每隔10分钟自动测量直肠温度。仪器偶尔出现故障导致数据系列出现相应的间断。通过计时图、综合图以及用24小时周期拟合、总体均值余弦分析法和线性-非线性最小二乘法分析计算得到的生物钟连续切片来检查数据。接下来，将单个余弦分析法得出的昼夜节律参数与一个周期等于相应月经间期天数的余弦曲线进行拟合。接着计算二阶亚昼夜推断统计量；月经第一天用作峰相位参考。月经间期的总体均值余弦分析法得出温度峰相位为-279度，95%置信区间从-254度延伸至-312度。由于不同个体之间和/或同一女性不同月经周期的月经间期不同，在不同情况下，这个峰相位对应月经第一天的不同时间间隔。因此，这个峰相位表明了直肠温度总体处于高位时在月经周期内的相对时间。在4名处于月经周期四个阶段的女性中，还昼夜每隔2小时采集一次血浆。测定了10种激素。尽管沿月经周期进行的内分泌采样稀疏，但在一名26岁女性中，所研究的所有激素均显示出30天节律。在与月经间期相对应的时期，T3、皮质醇、促卵泡激素、睾酮、硫酸脱氢表雄酮、T4和促黄体生成素的峰相位出现在30天直肠温度峰相位之前，而催乳素、雌二醇和孕酮的峰相位出现在直肠温度峰相位附近或之后不久。虽然早期对成年女性的30天节律映射是基于群体进行总结的，但本研究允许进行个体化的30天节律评估。30天节律与昼夜节律和年节律一样，峰相位和振幅关系不一定意味着因果关系，但它们是研究基本机制以及诊断和干预的不可或缺的定量参考标准，包括在计划生育方面的努力，这可能需要考虑生物体多频率的动态变化。
