Yasuma M, Takasaki Y, Matsumoto K, Kodama A, Hashimoto H, Hirose S
Department of Internal Medicine, Juntendo University, School of Medicine, Tokyo, Japan.
J Rheumatol. 1990 Apr;17(4):469-75.
Free Sm was purified by gel filtration and anti-Sm affinity chromatography. Using this purified antigen, an ELISA for anti-Sm was performed. Three hundred and fifty patients with various rheumatic diseases were studied with respect to immunoglobulin classes of anti-Sm by ELISA. A high frequency of IgG anti-Sm was specifically detected in patients with systemic lupus erythematosus (SLE), but IgA and IgM anti-Sm showed a low frequency and also was detected in other diseases. In patients with SLE, anti-Sm significantly correlated with lung fibrosis and pericarditis. In our longitudinal study, there were increases in titer of IgG anti-Sm preceding central nervous system exacerbation and serositis. IgG anti-Sm was found to be not only a diagnostic marker but also a reliable measure of disease activity in SLE.
通过凝胶过滤和抗Sm亲和层析法纯化游离Sm。使用这种纯化的抗原，进行了抗Sm的酶联免疫吸附测定（ELISA）。通过ELISA对350例患有各种风湿性疾病的患者的抗Sm免疫球蛋白类别进行了研究。在系统性红斑狼疮（SLE）患者中特异性检测到高频率的IgG抗Sm，但IgA和IgM抗Sm频率较低，且在其他疾病中也有检测到。在SLE患者中，抗Sm与肺纤维化和心包炎显著相关。在我们的纵向研究中，在中枢神经系统加重和浆膜炎之前，IgG抗Sm滴度升高。发现IgG抗Sm不仅是一种诊断标志物，也是SLE疾病活动的可靠指标。
