具有完全生物相容性的自组装生育酚-白蛋白纳米粒子用于乳腺癌的化学-光热治疗。
Self-Assembled Tocopherol-Albumin Nanoparticles with Full Biocompatibility for Chemo-photothermal Therapy against Breast Cancer.
机构信息
Department of Preventive Medicine and Public Health Laboratory Science, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, China.
Department of Thyroid and Breast Surgery, The Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu 212001, China.
出版信息
Curr Drug Deliv. 2022;19(1):49-63. doi: 10.2174/1567201819666211229120611.
BACKGROUND
The combination of photothermal therapy (PTT) and chemotherapy has proven to be a promising strategy for cancer treatment. Various nanomaterials have shown great potential in combination therapy, including gold, graphene oxide, iron oxide, and other nanoparticles. However, their undefinable toxicity greatly slowed down their development for clinical applications.
OBJECTIVE
The present work aimed to develop a multifunctional nanoparticle for chemo-photothermal therapy composed of acknowledged biocompatible materials.
METHODS
A novel biocompatible nanoparticle (HIT-NPs) was self-assembled through the intrinsic interaction between D-α-tocopherol Succinate (TOS), human serum albumin (HSA) and indocyanine green (ICG). Doxorubicin (DOX) was then loaded due to the ion pairing between DOX and TOS. The feasibility of combined chemo-photothermal therapy induced by DOX-loaded HIT-NPs was carefully evaluated.
RESULTS
, HIT-NPs showed no cytotoxicity on human normal liver cells (HL-7702 cells) but obvious killing effects on murine breast cancer cells (4T1 cells). The combined chemo-photothermal therapeutic effect on 4T1 cells was successfully obtained. DOX-loaded HIT-NPs could effectively accumulate in 4T1 subcutaneous tumors after intravenous injection, and the tumor temperature rapidly increased under laser exposure, indicating the feasibility of PTT .
CONCLUSION
The self-assembled HIT-NPs could provide a promising platform for combined chemo- photothermal cancer therapy with full biocompatibility.
背景
光热疗法（PTT）与化学疗法的联合已被证明是癌症治疗的一种很有前途的策略。各种纳米材料在联合治疗中显示出巨大的潜力，包括金、氧化石墨烯、氧化铁和其他纳米颗粒。然而，它们无法确定的毒性极大地减缓了它们在临床应用中的发展。
目的
本工作旨在开发一种由公认的生物相容性材料组成的用于化学-光热治疗的多功能纳米粒子。
方法
通过 D-α-生育酚琥珀酸（TOS）、人血清白蛋白（HSA）和吲哚菁绿（ICG）之间的固有相互作用，自组装了一种新型的生物相容性纳米粒子（HIT-NPs）。由于 DOX 和 TOS 之间的离子配对，随后加载了多柔比星（DOX）。仔细评估了由负载 DOX 的 HIT-NPs 诱导的联合化学-光热治疗的可行性。
结果
负载 DOX 的 HIT-NPs 对人正常肝细胞（HL-7702 细胞）没有细胞毒性，但对小鼠乳腺癌细胞（4T1 细胞）有明显的杀伤作用。成功获得了对 4T1 细胞的联合化学-光热治疗效果。负载 DOX 的 HIT-NPs 经静脉注射后可有效积聚在 4T1 皮下肿瘤中，并且在激光照射下肿瘤温度迅速升高，表明 PTT 的可行性。
结论
自组装的 HIT-NPs 可为具有完全生物相容性的联合化学-光热癌症治疗提供有前途的平台。
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