Department of Biochemistry, Health Science University, Bakirkoy Dr. Sadi Konuk Training and Research Hospital, İstanbul-Turkey.
Department of Pathology, Health Science University, Bakirkoy Dr. Sadi Konuk Training and Research Hospital, İstanbul-Turkey.
Ulus Travma Acil Cerrahi Derg. 2022 Jan;28(1):1-7. doi: 10.14744/tjtes.2020.29015.
We aimed to diagnose possible acute kidney injury (AKI) with new early biochemical markers in patients who were admitted to the emergency department frequently with mild and moderate brain trauma, and to prevent possible complications, shorten the duration of treatment and hospital stay. With this purpose, we decided to reach our scientific target using the experimental rat model.
Wistar albino rats were included our experiment. Fifteen rats were randomly separated into three groups: Sham control (n=1: Underwent craniotomy alone), control (n=7: Without craniotomy), and trauma group (n=7: Underwent craniotomy followed by brain injury).
There were no significant differences groups creatinine levels within 0 and 24 h (0.35±0.02 and 0.33±0.03, respectively, p>0.05). Plasma NGAL and KIM1 concentrations were statistically significant different in both control and trauma groups (Friedman p<0.05) and significant differences at both NGAL and KIM-1 concentrations at dual comparisons by means of all sampling time (0-2 h, 0-24 h, and 2-24 h) (Wilcoxon p<0.001, after Bonferroni correction).
The presence of AKI in patients with mild-to-moderate brain trauma increases the risk of mortality. Early diagnosis of AKI reduces the hospitalization period and requiring of dialysis. Diagnosis of AKI within 24 h with early biomarkers and starting therapy is crucial issues.
我们旨在通过新的早期生化标志物诊断经常因轻度和中度脑外伤而就诊于急诊科的患者中可能存在的急性肾损伤 (AKI)，并预防可能的并发症，缩短治疗和住院时间。为此，我们决定使用实验大鼠模型来实现我们的科学目标。
我们将 Wistar 白化大鼠纳入本实验。15 只大鼠被随机分为三组：假手术对照组（n=1：仅行开颅术）、对照组（n=7：不行开颅术）和创伤组（n=7：行开颅术后继发脑损伤）。
0 小时和 24 小时时各组肌酐水平无显著差异（分别为 0.35±0.02 和 0.33±0.03，p>0.05）。在对照组和创伤组中，血浆 NGAL 和 KIM1 浓度均有统计学差异（Friedman p<0.05），并且在所有采样时间（0-2 小时、0-24 小时和 2-24 小时）的双重比较中，NGAL 和 KIM-1 浓度均有显著差异（Wilcoxon p<0.001，经 Bonferroni 校正后）。
轻度至中度脑外伤患者中 AKI 的存在增加了死亡率。早期诊断 AKI 可缩短住院时间并减少透析的需要。在 24 小时内通过早期生物标志物进行 AKI 诊断并开始治疗是至关重要的问题。
