Thornburg W, Rao R K, Matrisian L M, Magun B E, Koldovský O
Am J Physiol. 1987 Jul;253(1 Pt 1):G68-71. doi: 10.1152/ajpgi.1987.253.1.G68.
Epidermal growth factor (EGF) was iodinated and administered orally to 13- to 15-day-old suckling rats and 29- to 31-day-old weanling rats. After 30 min, stomach, small intestine, plasma, liver, lung, and skin were removed. The tissues were homogenized and 125I radioactivity was extracted. Compared with suckling rats, the delivery of total radioactivity into peripheral tissues was enhanced in skin of weanling rats and tended to be higher in plasma and liver. In contrast, there was a 3.3-fold reduction in radioactivity remaining in the intestinal wall. Sephadex G-25 chromatography of most samples, especially liver and intestinal wall, revealed a decrease in the proportion of intact 125I-EGF eluting in the void volume. As a result, because the amount of total radioactivity also differed, the overall recovery of radioactivity of void volume 125I-EGF was similar in both age groups except for an increase in skin and a decrease in the intestinal wall of weanling rats. Extracts of all tissues of weanling rats examined contained immunoreactive 125I-EGF. Samples obtained from tissues and content of the gastrointestinal tract of both age groups bound specifically to A431 cell surface receptors. These results thus indicate that EGF is absorbed and delivered to various tissues of weanling rats. Nevertheless, quantitative and qualitative changes in these processes occur during the postnatal period.
将表皮生长因子（EGF）进行碘化处理后，给13至15日龄的乳鼠和29至31日龄的断奶大鼠口服。30分钟后，取出胃、小肠、血浆、肝脏、肺和皮肤。将组织匀浆并提取125I放射性。与乳鼠相比，断奶大鼠皮肤中外周组织的总放射性传递增强，血浆和肝脏中的总放射性传递有升高趋势。相反，肠壁中残留的放射性降低了3.3倍。大多数样品（尤其是肝脏和肠壁）的葡聚糖凝胶G - 25色谱分析显示，在空体积中洗脱的完整125I - EGF比例降低。因此，由于总放射性量也不同，除了断奶大鼠皮肤中增加和肠壁中减少外，两个年龄组中空体积125I - EGF的放射性总体回收率相似。所检测的断奶大鼠所有组织提取物均含有免疫反应性125I - EGF。两个年龄组从胃肠道组织和内容物中获得的样品均与A431细胞表面受体特异性结合。因此，这些结果表明EGF被吸收并传递到断奶大鼠的各种组织中。然而，在出生后时期，这些过程会发生定量和定性变化。
