Ovicidal activity of the hydroalcoholic extract of Brazilian peppertree ( Raddi) against spp. from naturally parasitized dogs.

This study aimed to evaluate the ovicidal activity of the hydroalcoholic extract of (SCH; T1) against spp. and its influence of storage time in the extract stored for 36 months (T36). Eggs of spp. were obtained from naturally parasitized dogs, and used for the larval hatchability test, where the eggs were exposed to T1 and T36 extracts of SCH (15-0.625 mg/mL). In T1, all concentrations inhibited more than 80% of the eggs, being 100% at concentrations between 15 and 5 mg/mL ( > 0.05). At T36, all concentrations were active, even the ones between 2.5 and 0.625 mg/mL, with 100% inhibition ( < 0.05), revealing that the storage time maintained the ovicidal action. By LC-MS, T36 presented ethyl gallate, myricitrin, and gallic acid as major compounds. These findings support the promising use of SCH extract as an ovicide against spp., even stored for 36 months of shelf life.