Smith S M, Holm-Rutili L, Perry M A, Grisham M B, Arfors K E, Granger D N, Kvietys P R
Gastroenterology. 1987 Sep;93(3):466-71. doi: 10.1016/0016-5085(87)90907-3.
Gastric mucosal clearance of 51Cr-labeled red blood cells (51Cr-RBC) was measured in rats during a 30-min control period, a 30-min ischemic period (hemorrhage to 27 mmHg arterial pressure), and a 60-min reperfusion period (reinfusion of shed blood). In untreated (control) rats, a dramatic rise in the leakage of 51Cr-labeled red blood cells into the gastric lumen was observed during the reperfusion period. Treatment with neutrophil antiserum attenuated 51Cr-labeled red blood cell flux into the gastric lumen. Using the radioactive microsphere technique, neutrophil-depleted animals were shown to have higher blood flows in the ischemic period than the untreated rats. Bleeding of untreated rats to a mean arterial pressure of 40 mmHg resulted in blood flows that were not different from those in antiserum-treated rats bled to 27 mmHg and leakage of 51Cr-labeled red blood cells similar to that measured in antiserum-treated rats. The results of this study indicate that neutrophils play an important role in hemorrhagic shock-induced gastric bleeding.
在大鼠中测量了51铬标记的红细胞（51Cr-RBC）的胃黏膜清除率，测量时段包括30分钟的对照期、30分钟的缺血期（动脉血压降至27 mmHg）以及60分钟的再灌注期（回输 shed blood）。在未处理（对照）的大鼠中，在再灌注期观察到51Cr标记的红细胞向胃腔内渗漏显著增加。用中性粒细胞抗血清处理可减弱51Cr标记的红细胞流入胃腔的通量。使用放射性微球技术显示，中性粒细胞减少的动物在缺血期的血流量高于未处理的大鼠。将未处理大鼠的血压降至平均动脉压40 mmHg，其血流量与抗血清处理的大鼠降至27 mmHg时的血流量无差异，且51Cr标记的红细胞渗漏情况与抗血清处理的大鼠相似。本研究结果表明，中性粒细胞在失血性休克诱导的胃出血中起重要作用。
