Sakanaka M, Shibasaki T, Lederis K
J Comp Neurol. 1987 Jun 8;260(2):256-98. doi: 10.1002/cne.902600209.
A cobalt-glucose-oxidase diaminobenzidine (Co-GOD) method, employing a specific antiserum against rat corticotropin releasing factor (CRF), was applied to determine immunohistochemically a widespread and detailed localization of corticotropin releasing factor-like immunoreactivity (CRFI) in the rat brain. Besides the CRFI cells in the paraventricular hypothalamic nucleus that project to the median eminence, CRFI cells were demonstrated in many brain regions, including the olfactory bulb, cerebral cortex, septal nuclei, hippocampus, amygdala, thalamic nuclei, medial hypothalamic nuclei, lateral hypothalamic area, perifornical area, central gray, cuneiform nucleus, inferior colliculus, raphe nuclei, mesencephalic reticular formation, laterodorsal tegmental nucleus, locus coeruleus, parabrachial nuclei, mesencephalic tract of the trigeminal nerve, pontine reticular formation, lateral superior olive, vestibular nuclei, prepositus hypoglossal nucleus, nucleus of the solitary tract, dorsal motor nucleus of the vagus, lateral reticular nucleus, nucleus of the spinal tract of the trigeminal nerve, external cuneate nucleus, inferior olive, and medullary reticular formation. CRFI-reacting neural processes were also detected in these same areas. In particular, the median eminence, lateral septum, bed nucleus of the stria terminalis, mesencephalic reticular formation, parabrachial nuclei, and nucleus of the solitary tract contained large numbers of CRFI fibres. The widespread localization of CRFI demonstrated in the present study strongly suggests that CRF, like many other neurohormones and peptides, may act as a neurotransmitter and/or neuromodulator in numerous extrahypothalamic circuits, as well as participate in neuroendocrine regulation.
采用一种钴 - 葡萄糖氧化酶 - 二氨基联苯胺（Co - GOD）方法，运用针对大鼠促肾上腺皮质激素释放因子（CRF）的特异性抗血清，通过免疫组织化学法来确定大鼠脑中促肾上腺皮质激素释放因子样免疫反应性（CRFI）广泛而详细的定位。除了投射到正中隆起的下丘脑室旁核中的CRFI细胞外，在许多脑区都发现了CRFI细胞，包括嗅球、大脑皮层、隔核、海马体、杏仁核、丘脑核、下丘脑内侧核、下丘脑外侧区、穹窿周区、中央灰质、楔形核、下丘、中缝核、中脑网状结构、外侧背盖核、蓝斑、臂旁核、三叉神经中脑束、脑桥网状结构、外侧上橄榄核、前庭核、舌下前置核、孤束核、迷走神经背运动核、外侧网状核、三叉神经脊束核、楔外核、下橄榄核和延髓网状结构。在这些相同区域也检测到了CRFI反应性神经突起。特别是，正中隆起、外侧隔、终纹床核、中脑网状结构、臂旁核和孤束核含有大量CRFI纤维。本研究中所展示的CRFI的广泛定位强烈表明，CRF与许多其他神经激素和肽一样，可能在众多下丘脑外神经回路中作为神经递质和/或神经调节剂发挥作用，同时也参与神经内分泌调节。
