Chang Ying, Jiao Yan, Li Da-Jing, Liu Xiao-Lan, Han He
College of Food and Bioengineering, Qiqihar University, Qiqihar 161006, China.
College of Food and Bioengineering, Qiqihar University, Qiqihar 161006, China.
Food Chem. 2021 Dec 23;376:131927. doi: 10.1016/j.foodchem.2021.131927.
Glucosamine-glycosylated zein (GLZ) generated by transglutaminase was developed as a novel delivery vehicle to prepare lutein-loaded glycosylated zein nanoparticles (GLZ-LUT). GLZ-LUT exhibited a polydispersed spherical microstructure, lutein was embedded into GLZ to form nanocomplexes via self-assembly, they had a lower zeta potential and an average particle size of less than 200 nm. Compared to lutein-loaded zein nanoparticles (Zein-LUT), the lutein entrapment efficiency of GLZ-LUT was increased from 81.55% to 89.60%. Infrared spectroscopy (FTIR) analysis results confirmed that zein was successfully modified and that lutein was encapsulated by hydrophobic zein and GLZ. Moreover, GLZ showed significantly higher solubilization of lutein than Zein-LUT and significantly improved the in vitro release of lutein in the simulated gastrointestinal tract. The in vitro antioxidant activity of lutein was also enhanced by the encapsulation of zein and glycosylated zein. These findings indicated that GLZ represent a potentially efficient and promising nanodelivery carrier for lutein compounds.
通过转谷氨酰胺酶生成的葡萄糖胺糖基化玉米醇溶蛋白（GLZ）被开发为一种新型递送载体，用于制备负载叶黄素的糖基化玉米醇溶蛋白纳米颗粒（GLZ-LUT）。GLZ-LUT呈现出多分散的球形微观结构，叶黄素通过自组装嵌入GLZ中形成纳米复合物，它们具有较低的zeta电位，平均粒径小于200 nm。与负载叶黄素的玉米醇溶蛋白纳米颗粒（Zein-LUT）相比，GLZ-LUT中叶黄素的包封率从81.55%提高到了89.60%。红外光谱（FTIR）分析结果证实玉米醇溶蛋白已成功改性，且叶黄素被疏水性玉米醇溶蛋白和GLZ包封。此外，GLZ对叶黄素的增溶作用明显高于Zein-LUT，并显著改善了叶黄素在模拟胃肠道中的体外释放。玉米醇溶蛋白和糖基化玉米醇溶蛋白的包封还增强了叶黄素的体外抗氧化活性。这些发现表明，GLZ是一种用于叶黄素化合物的潜在高效且有前景的纳米递送载体。
