Zhan Feili, Luo Jiafeng, Sun Yangying, Hu Yangyang, Fan Xiankang, Pan Daodong
Key Laboratory of Animal Protein Food Processing Technology of Zhejiang Province, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315211, China.
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Ningbo University, Ningbo 315211, China.
Foods. 2023 Jan 26;12(3):540. doi: 10.3390/foods12030540.
Duck liver is an important by-product of duck food. In this study, we investigated the effects of glucose, fructose, and xylose on the antioxidant properties of glycosylated products of duck liver protein and their protective effects on HepG2 cells. The results show that the glycosylation products of the three duck liver proteins (DLP-G, DLP-F, and DLP-X) all exhibit strong antioxidant activity; among three groups, DLP-X shows the strongest ability to scavenge DPPH, ·OH free radicals, and ABTS free radicals. The glycosylated products of duck liver protein are not toxic to HepG2 cells and significantly increase the activity of antioxidant enzymes such as SOD, CAT, and GSH-Px in HepG2 cells at the concentration of 2.0 g/L, reducing oxidative stress damage of cells ( < 0.05). DLP-X has a better effect in reducing oxidative damage and increasing cellular activity in HepG2 cells than DLP-G and DLP-F ( < 0.05). In this study, the duck liver protein glycosylated products by glucose, fructose, and xylose were named as DLP-G, DLP-F, and DLP-X, respectively.
鸭肝是鸭类食品的一种重要副产品。在本研究中，我们研究了葡萄糖、果糖和木糖对鸭肝蛋白糖基化产物抗氧化性能的影响及其对HepG2细胞的保护作用。结果表明，三种鸭肝蛋白糖基化产物（DLP-G、DLP-F和DLP-X）均表现出较强的抗氧化活性；在三组中，DLP-X清除DPPH、·OH自由基和ABTS自由基的能力最强。鸭肝蛋白糖基化产物对HepG2细胞无毒，在2.0 g/L浓度下能显著提高HepG2细胞中SOD、CAT和GSH-Px等抗氧化酶的活性，减轻细胞的氧化应激损伤（P<0.05）。DLP-X在减轻HepG2细胞氧化损伤和提高细胞活性方面比DLP-G和DLP-F效果更好（P<0.05）。在本研究中，由葡萄糖、果糖和木糖生成的鸭肝蛋白糖基化产物分别命名为DLP-G、DLP-F和DLP-X。
