评估16T静磁场与700MHz射频电磁场对小鼠的生物安全性。
Evaluating the biological safety on mice at 16 T static magnetic field with 700 MHz radio-frequency electromagnetic field.
作者信息
Wang Shenghang, Zheng Mengxuan, Lou Chenge, Chen Shuai, Guo Huijie, Gao Yang, Lv Huanhuan, Yuan Xichen, Zhang Xiaotong, Shang Peng
机构信息
Research & Development Institute of Northwestern Polytechnical University in Shenzhen, Shenzhen, China; School of Life Sciences, Northwestern Polytechnical University, Xi'an, Shaanxi, China; Key Laboratory for Space Biosciences and Biotechnology, Northwestern Polytechnical University, Xi'an, Shaanxi, China.
Key Laboratory for Biomedical Engineering of Ministry of Education, College of Biomedical Engineering & Instrument Science, Zhejiang University, Hangzhou, China.
出版信息
Ecotoxicol Environ Saf. 2022 Jan 15;230:113125. doi: 10.1016/j.ecoenv.2021.113125. Epub 2021 Dec 28.
OBJECTIVES
This study evaluated the associated biological effects of radio-frequency (RF) exposure at 16 T magnetic resonance imaging (MRI) on mice health.
MATERIAL AND METHODS
A total of 48 healthy 8-week-old male C57BL/6 mice were investigated. A 16 T high static magnetic field (HiSMF) was generated by a superconducting magnet, and a radiofrequency (RF) electromagnetic field for hydrogen resonance at 16 T (700 MHz) was transmitted via a homemade RF system. The mice were exposed inside the 16 T HiSMF with the 700 MHz RF field for 60 min, and the body weight, organ coefficients, histomorphology of major organs, and blood indices were analyzed for the basal state of the mice on day 0 and day 14. The Heat Shock Protein 70 (HSP70), cyclooxygenase 2 (COX2), and interleukin- 6 (IL-6) were used to evaluate the thermal effects on the brain. Locomotor activity, the open field test, tail suspension test, forced swimming test, and grip strength test were used to assess the behavioral characteristics of the mice.
RESULTS
The 16 T HiSMF with 700 MHz RF electromagnetic field exposure had no significant effects on body weight, organ coefficients, or histomorphology of major organs in the mice. On day 0, the expressions of HSP70 and COX2 in the brain were increased by 16 T HiSMF with 700 MHz RF electromagnetic field exposure. However, the expression of HSP70, COX2, and IL-6 had no significant difference compared with the sham group on day 14. Compared with the sham groups, the meancorpuscularvolume (MCV) on day 0 and the total protein (TP) on day 14 were increased significantly, whereas the other blood indices did not change significantly. The 16 T HiSMF with 700 MHz RF electromagnetic field exposure caused the mice to briefly circle tightly but had no effect on other behavioral indicators.
CONCLUSIONS
In summary, 16 T HiSMF with 700 MHz RF electromagnetic field exposure for 60 min did not have severe effects on mice.
目的
本研究评估了16T磁共振成像（MRI）时射频（RF）暴露对小鼠健康的相关生物学效应。
材料与方法
共研究了48只8周龄健康雄性C57BL/6小鼠。通过超导磁体产生16T高静磁场（HiSMF），并通过自制的射频系统传输用于16T（700MHz）氢共振的射频（RF）电磁场。将小鼠置于16T HiSMF内，暴露于700MHz射频场60分钟，并在第0天和第14天分析小鼠的基础状态下的体重、器官系数、主要器官的组织形态学和血液指标。使用热休克蛋白70（HSP70）、环氧化酶2（COX2）和白细胞介素-6（IL-6）评估对大脑的热效应。使用自主活动、旷场试验、悬尾试验、强迫游泳试验和握力试验评估小鼠的行为特征。
结果
暴露于700MHz射频电磁场的16T HiSMF对小鼠的体重、器官系数或主要器官的组织形态学没有显著影响。在第0天，暴露于700MHz射频电磁场的16T HiSMF使大脑中HSP70和COX2的表达增加。然而，在第14天，与假手术组相比，HSP70、COX2和IL-6的表达没有显著差异。与假手术组相比，第0天的平均红细胞体积（MCV）和第14天的总蛋白（TP）显著增加，而其他血液指标没有显著变化。暴露于700MHz射频电磁场的16T HiSMF使小鼠短暂地紧紧转圈，但对其他行为指标没有影响。
结论
总之，暴露于700MHz射频电磁场的16T HiSMF 60分钟对小鼠没有严重影响。
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