Division of Basic Radiation Bioscience, Korea Institute of Radiological & Medical Sciences, Seoul, Korea.
Division of Life Sciences, Korea University, Seoul, Korea.
Brain Behav. 2020 Nov;10(11):e01815. doi: 10.1002/brb3.1815. Epub 2020 Aug 28.
Due to public concerns about deleterious biological consequences of radiofrequency electromagnetic fields (RF-EMF), the potential effects of RF-EMF on the central nervous system have received wide consideration.
Here, two groups of C57BL/6 mice, aged 2 and 12 months, were exposed to 1,950-MHz RF-EMF at a specific absorption rate of 5.0 W/kg for chronic periods (2 hr/day and 5 days/week for 8 months). Behavioral changes were then assessed in the mice at 10 months (sham- or RF-10M) and 20 months (sham- or RF-20M), on the open-field test, the Y-maze test, and an object recognition memory task, while biological effects were analyzed via microarray gene profiling of the hippocampus.
Open-field test results showed a decrease in the time duration spent at the center while there was a decrease in enhanced memory shown by the Y-maze test and the novel object recognition test in the RF-20M mice, compared to sham-exposed mice, but no significant changes in the RF-10M group. Based on a 2-fold change cutoff, the microarray data revealed that 15 genes, which are listed as being involved in neurogenesis on Gene Ontology, were altered in both groups. Quantitative real-time PCR for validation showed increased expression of Epha8 and Wnt6 in the hippocampi of RF-20M group mice, although 13 additional genes showed no significant changes following RF-EMF exposure.
Therefore, cognitive enhancement following chronic exposure for 8 months to RF-EMF from middle age may be associated with increases in neurogenesis-related signals in the hippocampus of C57BL/6 mice.
由于公众对射频电磁场（RF-EMF）的有害生物学后果的担忧，RF-EMF 对中枢神经系统的潜在影响受到了广泛关注。
在这里，两组 2 个月和 12 个月大的 C57BL/6 小鼠，以 5.0 W/kg 的特定吸收率暴露于 1950MHz RF-EMF 中，进行慢性暴露（2 小时/天，每周 5 天，持续 8 个月）。然后在 10 个月（假暴露或 RF-10M）和 20 个月（假暴露或 RF-20M）时，通过旷场试验、Y 迷宫试验和物体识别记忆任务评估小鼠的行为变化，同时通过海马体的微阵列基因谱分析研究生物效应。
旷场试验结果显示，与假暴露组相比，RF-20M 组小鼠在中央区域的停留时间减少，Y 迷宫试验和新物体识别试验的记忆增强减少，但 RF-10M 组无明显变化。基于 2 倍变化的截止值，微阵列数据显示，在两组中，有 15 个基因（在基因本体论中被列为参与神经发生）发生改变。定量实时 PCR 验证显示，RF-20M 组小鼠海马中 EphA8 和 Wnt6 的表达增加，尽管 13 个其他基因在 RF-EMF 暴露后没有明显变化。
因此，从中年开始连续暴露 8 个月 RF-EMF 后认知增强可能与 C57BL/6 小鼠海马中与神经发生相关的信号增加有关。
