Department of Chemical Engineering, KTH Royal Institute of Technology, SE-100 44, Stockholm, Sweden.
Department of Chemical Engineering, KTH Royal Institute of Technology, SE-100 44, Stockholm, Sweden.
J Environ Manage. 2022 Mar 1;305:114337. doi: 10.1016/j.jenvman.2021.114337. Epub 2021 Dec 28.
Polyhydroxyalkanoates (PHAs), as bio-based plastics, promise a transition from petroleum products to green and sustainable alternatives. However, their commercial production is yet impeded by high production costs. In this study, we assessed synthetic culture in mono and co-culture modes for bacterial PHA production. It was demonstrated that volatile fatty acids (VFAs) derived from food waste and primary sludge are cheap carbon sources for maintaining high production yields in the synthetic cultures. The maximum obtained PHA was 77.54 ± 5.67% of cell dried weight (CDW) (1.723 g/L) from Cupriavidus necator and 54.9 ± 3.66% of CDW (1.088 g/L) from Burkholderia cepacia. The acquired results are comparable to those in literature using sugar substrates. Comparatively, lower PHA productions were obtained from the co-cultivations ranging between 36-45 CDW% (0.39-0.48 g/L). Meanwhile, the 3-hydroxyvalerate content in the biopolymers were increased up to 21%.
聚羟基烷酸酯（PHAs）作为生物基塑料，有望实现从石油产品向绿色可持续替代品的转变。然而，其商业生产仍受到高生产成本的阻碍。在本研究中，我们评估了细菌 PHA 生产的单一和混合培养的合成培养。结果表明，来自食物垃圾和初沉污泥的挥发性脂肪酸（VFAs）是维持合成培养中高生产产量的廉价碳源。从鞘氨醇单胞菌中获得的最大 PHA 为细胞干重（CDW）的 77.54±5.67%（1.723g/L），从洋葱伯克霍尔德氏菌中获得的最大 PHA 为 CDW 的 54.9±3.66%（1.088g/L）。所获得的结果与使用糖底物的文献中的结果相当。相比之下，从混合培养中获得的 PHA 产量较低，范围在 36-45 CDW%（0.39-0.48g/L）之间。同时，生物聚合物中的 3-羟基戊酸含量增加到 21%。
