Division of Applied Oral Sciences, Nihon University Graduate School of Dentistry, Tokyo, Japan.
Department of Periodontology, Nihon University School of Dentistry, Tokyo, Japan.
In Vivo. 2022 Jan-Feb;36(1):76-85. doi: 10.21873/invivo.12678.
BACKGROUND/AIM: Guided bone regeneration (GBR) is one of the surgical methods used for vertical ridge augmentation prior to dental implant placements. Titanium meshes have been used for osteogenic space maintenance in GBR sites by clinicians. We aimed to compare the influence of micropores and macropores in a titanium mesh on bone regeneration in a rat calvarial vertical GBR model.
The calvaria of nine rats were exposed, and plastic cylinders were set bilaterally. Eighteen surgical sites were randomly allocated into three groups according to the materials of titanium lid and bone substitutes: microporous titanium lid+deproteinized bovine bone mineral (DBBM), macroporous titanium lid +DBBM, microporous titanium lid+carbonate apatite. Newly generated bone inside the cylinders was evaluated using micro-computed tomography (micro-CT). Furthermore, bone regeneration and angiogenesis were evaluated histologically at 12 weeks.
Quantitative volumetric analyses using micro-CT showed a gradual increase in bone volume inside the cylinders in all three groups. Histological observation confirmed vigorous bone regeneration in the microporous groups compared to that in the macroporous group. In the upper part of the cylinders, soft tissue invaded the GBR site by passing through the pores of the macroporous mesh. The blood vessels in the upper part of the cylinders were smaller in the microporous groups than in the macroporous group. There was no difference in bone formation between cylinders filled with DBBM or carbonate apatite.
Microvasculature penetrates 50-μm diameter micropores and accelerates bone formation inside the cylinder, which was set on rat calvaria. The microporous titanium mesh can facilitate angiogenesis from both the dura mater and periosteal in vertical ridge augmentation. Our data showed superiority of microporous titanium vascular permeability and osteoconductivity, supporting bone growth.
背景/目的：引导骨再生（GBR）是在牙种植体植入前用于垂直牙槽嵴增加的一种手术方法。临床医生使用钛网来维持 GBR 部位的成骨空间。我们旨在比较钛网中微孔和大孔对大鼠颅骨垂直 GBR 模型中骨再生的影响。
暴露 9 只大鼠的颅骨，并在双侧设置塑料圆柱体。根据钛盖和骨替代物的材料，将 18 个手术部位随机分为三组：微孔钛盖+脱蛋白牛骨矿物质（DBBM）、大孔钛盖+DBBM、微孔钛盖+碳酸磷灰石。使用微计算机断层扫描（micro-CT）评估圆柱体内部新生成的骨。此外，在 12 周时通过组织学评估骨再生和血管生成。
使用 micro-CT 的定量体积分析显示，所有三组圆柱体内部的骨体积逐渐增加。组织学观察证实，与大孔组相比，微孔组有更活跃的骨再生。在圆柱体的上部，软组织通过大孔网的孔侵入 GBR 部位。圆柱体上部的血管在微孔组比大孔组小。填充 DBBM 或碳酸磷灰石的圆柱体之间的骨形成没有差异。
微血管穿透 50-μm 直径的微孔，并加速大鼠颅骨上设置的圆柱体内部的骨形成。微孔钛网可促进垂直嵴增加中硬脑膜和骨膜的血管生成。我们的数据显示出微孔钛血管通透性和骨传导性的优势，支持骨生长。
