Hartjen Philip, Hoffmann Alexia, Henningsen Anders, Barbeck Mike, Kopp Alexander, Kluwe Lan, Precht Clarissa, Quatela Olivia, Gaudin Robert, Heiland Max, Friedrich Reinhard E, Knipfer Christian, Grubeanu Daniel, Smeets Ralf, Jung Ole
Department of Oral and Maxillofacial Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
Meotec GmbH & Co. KG, Aachen, Germany.
In Vivo. 2018 Mar-Apr;32(2):241-247. doi: 10.21873/invivo.11230.
BACKGROUND/AIM: Plasma electrolytic oxidation (PEO) is an established electrochemical treatment technique that can be used for surface modifications of metal implants. In this study we to treated titanium implants with PEO, to examine the resulting microstructure and to characterize adhesion and viability of cells on the treated surfaces. Our aim was to identify an optimal surface-modification for titanium implants in order to improve soft-tissue integration.
Three surface-variants were generated on titanium alloy Ti6Al4V by PEO-treatment. The elemental composition and the microstructures of the surfaces were characterized using energy dispersive X-ray spectroscopy, scanning electron microscopy and profilometry. In vitro cytocompatibility of the surfaces was assessed by seeding L929 fibroblasts onto them and measuring the adhesion, viability and cytotoxicity of cells by means of live/dead staining, XTT assay and LDH assay.
Electron microscopy and profilometry revealed that the PEO-surface variants differed largely in microstructure/topography, porosity and roughness from the untreated control material as well as from one another. Roughness was generally increased after PEO-treatment. In vitro, PEO-treatment led to improved cellular adhesion and viability of cells accompanied by decreased cytotoxicity.
PEO-treatment provides a promising strategy to improve the integration of titanium implants with surrounding tissues.
背景/目的：等离子体电解氧化（PEO）是一种成熟的电化学处理技术，可用于金属植入物的表面改性。在本研究中，我们对钛植入物进行了PEO处理，以检查由此产生的微观结构，并表征处理过的表面上细胞的粘附和活力。我们的目的是确定钛植入物的最佳表面改性方法，以改善软组织整合。
通过PEO处理在钛合金Ti6Al4V上生成三种表面变体。使用能量色散X射线光谱、扫描电子显微镜和轮廓仪对表面的元素组成和微观结构进行表征。通过将L929成纤维细胞接种到表面上，并通过活/死染色、XTT测定和LDH测定来测量细胞的粘附、活力和细胞毒性，评估表面的体外细胞相容性。
电子显微镜和轮廓仪显示，PEO表面变体在微观结构/形貌、孔隙率和粗糙度方面与未处理的对照材料以及彼此之间有很大差异。PEO处理后粗糙度通常会增加。在体外，PEO处理导致细胞粘附和活力提高，同时细胞毒性降低。
PEO处理为改善钛植入物与周围组织的整合提供了一种有前景的策略。
