Cell Cycle and Cancer Biology Research program, Oklahoma Medical Research Foundation, Oklahoma City, OK, USA.
Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX, USA.
Methods Mol Biol. 2022;2415:61-86. doi: 10.1007/978-1-0716-1904-9_5.
Live-cell fluorescence microscopy is an effective tool for characterizing aberrant mitotic phenotypes resulting from exposure to chemical inhibitors and after RNA interference-mediated or CRISPR knockout-mediated depletion of protein targets. Live imaging of cultured cells during mitotic progression presents challenges in maintaining optimal health of cells while achieving the temporal and spatial resolution to accomplish the goals of the study. Herein are strategies to monitor and analyze mammalian cell mitosis utilizing either a wide field or a light sheet, inverted, fluorescence microscope.
活细胞荧光显微镜是一种有效的工具，可用于表征因暴露于化学抑制剂以及 RNA 干扰介导或 CRISPR 敲除介导的蛋白靶标耗竭而导致的有丝分裂表型异常。在有丝分裂进程中对培养细胞进行实时成像，存在着维持细胞最佳健康状态的挑战，同时还需要实现时间和空间分辨率，以完成研究目标。本文介绍了使用宽场或光片倒置荧光显微镜来监测和分析哺乳动物细胞有丝分裂的策略。
