用于有丝分裂表型分析的活细胞荧光成像
Live-cell fluorescence imaging for phenotypic analysis of mitosis.
作者信息
Sivakumar Sushama, Daum John R, Gorbsky Gary J
机构信息
Cell Cycle and Cancer Biology Research Program, Oklahoma Medical Research Foundation, 825 NE 13th Street, MS 48, Oklahoma City, OK, 73104, USA.
出版信息
Methods Mol Biol. 2014;1170:549-62. doi: 10.1007/978-1-4939-0888-2_31.
Abstract
Live-cell fluorescence microscopy is a powerful tool for characterizing aberrant mitotic phenotypes resulting from exposure to chemical inhibitors or after depletion of protein targets by RNA interference or other methods. Live imaging of cultured cells during mitotic progression presents challenges in maintaining optimal health of cells while achieving the temporal and spatial resolution to accomplish the goals of the study. We describe herein strategies to monitor and analyze mammalian cell mitosis with standard, inverted, fluorescence microscopy systems that are widely available.
摘要
活细胞荧光显微镜术是一种强大的工具，可用于表征因接触化学抑制剂或通过RNA干扰或其他方法耗尽蛋白质靶点后所产生的异常有丝分裂表型。在有丝分裂进程中对培养细胞进行实时成像，在维持细胞的最佳健康状态同时实现时间和空间分辨率以完成研究目标方面存在挑战。我们在此描述了使用广泛可用的标准倒置荧光显微镜系统监测和分析哺乳动物细胞有丝分裂的策略。
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