同步哺乳动物细胞以进行生存素定位的有丝分裂分析。
Synchronizing Mammalian Cells for Mitotic Analysis of the Localization of Survivin.
作者信息
Wheatley Sally P
机构信息
School of Life Sciences, University of Nottingham, Medical School, Queen's Medical Centre, Nottingham, UK.
出版信息
Methods Mol Biol. 2022;2415:95-103. doi: 10.1007/978-1-0716-1904-9_7.
PMID:34972948
Abstract
This paper describes an easy method to enrich the harvest of adherent mammalian cells at each stage of mitosis (from prometaphase to cytokinesis) by combining Eg5 inhibition using dimethylenastron (DMA) with mitotic shake-off, followed by timed release from the drug.
摘要
本文描述了一种简便方法，通过将使用二甲苯磺酸钠（DMA）抑制驱动蛋白 Eg5 与有丝分裂摇落法相结合，然后定时从药物中释放，在有丝分裂的每个阶段（从前中期到胞质分裂）富集贴壁哺乳动物细胞收获物。
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