染色体不稳定的核生物标志物检测

Detection of Nuclear Biomarkers for Chromosomal Instability.

作者信息

Pons Carles, Almacellas Eugenia, Tauler Albert, Mauvezin Caroline

机构信息

Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute for Science and Technology, Barcelona, Catalonia, Spain.

Molecular Cell Biology of Autophagy, The Francis Crick Institute, London, UK.

出版信息

Methods Mol Biol. 2022;2445:117-125. doi: 10.1007/978-1-0716-2071-7_8.

DOI:10.1007/978-1-0716-2071-7_8

PMID:34972989

Abstract

Chromosomal instability (CIN) is a hallmark of cancer, which is characterized by the gain or loss of chromosomes as well as the rearrangement of the genetic material during cell division. Detection of mitotic errors such as misaligned chromosomes or chromosomal bridges (also known as lagging chromosomes) is challenging as it requires the analysis and manual discrimination of chromosomal aberrations in mitotic cells by molecular techniques. In interphase cells, more frequent in the cell population than mitotic cells, two distinct nuclear phenotypes are associated with CIN: the micronucleus and the toroidal nucleus. Several methods are available for the detection of micronuclei, but none for toroidal nuclei. Here, we provide a method to quantify the presence of both nuclear biomarkers for the evaluation of CIN status in non-mitotic cells particularly suited for genotoxicity screens.

摘要

染色体不稳定（CIN）是癌症的一个标志，其特征是在细胞分裂过程中出现染色体的增减以及遗传物质的重排。检测有丝分裂错误，如染色体排列不齐或染色体桥（也称为落后染色体）具有挑战性，因为这需要通过分子技术对有丝分裂细胞中的染色体畸变进行分析和人工鉴别。在间期细胞（在细胞群体中比有丝分裂细胞更常见）中，有两种不同的核表型与CIN相关：微核和环形核。有几种方法可用于检测微核，但尚无用于检测环形核的方法。在此，我们提供一种方法来量化这两种核生物标志物的存在情况，以评估非有丝分裂细胞中的CIN状态，该方法特别适用于遗传毒性筛选。