Department of Endodontics, The Nippon Dental University School of Life Dentistry at Tokyo, Tokyo, 102-8159, Japan.
Department of Biochemistry, The Nippon Dental University School of Life Dentistry at Tokyo, Tokyo, 102-8159, Japan.
Biochem Biophys Res Commun. 2022 Jan 29;590:68-74. doi: 10.1016/j.bbrc.2021.12.093. Epub 2021 Dec 27.
The mutation and deletion of high mobility group AT-hook 2 (Hmga2) gene exhibit skeletal malformation, but almost nothing is known about the mechanism. This study examined morphological anomaly of facial bone in Hmga2 mice and osteoblast differentiation of pre-osteoblast MC3T3-E1 cells with Hmga2 gene knockout (A2KO). Hmga2 mice showed the size reduction of anterior frontal part of facial bones. Hmga2 protein and mRNA were expressed in mesenchymal cells at ossification area of nasal bone. A2KO cells differentiation into osteoblasts after reaching the proliferation plateau was strongly suppressed by alizarin red and alkaline phosphatase staining analyses. Expression of osteoblast-related genes, especially Osterix, was down-regulated in A2KO cells. These results demonstrate a close association of Hmga2 with osteoblast differentiation of mesenchymal cells and bone growth. Although future studies are needed, the present study suggests an involvement of Hmga2 in osteoblast-genesis and bone growth.
高迁移率族蛋白 A2（Hmga2）基因突变和缺失会导致骨骼畸形，但目前对于其机制几乎一无所知。本研究通过观察 Hmga2 基因敲除（A2KO）小鼠的面部骨形态异常和前成骨细胞 MC3T3-E1 细胞的成骨细胞分化，研究 Hmga2 基因的作用。Hmga2 小鼠的面部骨前额部分明显变小。Hmga2 蛋白和 mRNA 在鼻骨成骨区的间充质细胞中表达。A2KO 细胞在达到增殖平台后向成骨细胞分化的能力受到茜素红和碱性磷酸酶染色分析的强烈抑制。A2KO 细胞中骨相关基因的表达，特别是 Osterix，下调。这些结果表明 Hmga2 与间充质细胞的成骨细胞分化和骨生长密切相关。尽管需要进一步研究，但本研究提示 Hmga2 可能参与成骨细胞生成和骨生长。
